|
| Status |
Public on Jun 07, 2016 |
| Title |
Ribosomal footprinting of CN34-Parental and CN34-LM1a |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
We used Illlumina Artseq Ribo Profile to perform ribosome footprinting on the above cell lines
|
| |
|
| Overall design |
We followed the manufacturer's protocol: Cells were treated with cycloheximide (0.1 mg/ml) to inhibit translation; total RNA(tt) and ribosome-protected RNA fragments (RPF) were isolated and library preped for sequencing simultaneously.
|
| |
|
| Contributor(s) |
Goodarzi H, Nguyen H, Tavazoie SF |
| Citation(s) |
27259150 |
| Submission date |
Jan 27, 2016 |
| Last update date |
May 15, 2019 |
| Contact name |
Hani Goodarzi |
| Organization name |
UCSF
|
| Department |
Biochemistry and Biophysics
|
| Street address |
600 16th St, GH S312D
|
| City |
San Francisco |
| State/province |
CA |
| ZIP/Postal code |
94158 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL18573 |
Illumina NextSeq 500 (Homo sapiens) |
|
| Samples (8)
|
|
| This SubSeries is part of SuperSeries: |
| GSE77401 |
Modulated expression of specific tRNA species drives cancer progression |
|
| Relations |
| BioProject |
PRJNA309995 |
| SRA |
SRP068967 |
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