|
| Status |
Public on Feb 11, 2017 |
| Title |
A RUNX2-mediated Epigenetic Regulation of the Survival of p53 Defective Cancer Cells |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
The goal is to identify transcripts that are regulated by RUNX2 in SAOS2 cells.
|
| |
|
| Overall design |
SAOS2 cells were transduced with lentiviruses expressing shLuc, shRUNX2_3 and shRUNX2_4 for 4 days followed by 2ug/ul puromycin selection to remove untransduced cells. A no-virus control was included to monitor the transduction efficiency. These control cells died after 2 days of puromycin selection. The lentivirally transduced cells were more than 80% survived, which indicates a high transduction efficiency. After four more days in puromycin, SAOS2 cells were harvested and subjected to total RNA extraction. 1ug total RNA were used to perform RNA-seq.
|
| |
|
| Contributor(s) |
Huang J |
| Citation(s) |
26925584 |
| Submission date |
Jan 28, 2016 |
| Last update date |
May 15, 2019 |
| Contact name |
Jing Huang |
| E-mail(s) |
huangj3@mail.nih.gov
|
| Organization name |
National Cancer Institute
|
| Lab |
Cancer Biology and Genetics
|
| Street address |
37 Convent Dr. 37/3140
|
| City |
Bethesda |
| State/province |
MD |
| ZIP/Postal code |
MD 20892 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL18573 |
Illumina NextSeq 500 (Homo sapiens) |
|
| Samples (9)
|
|
| Relations |
| BioProject |
PRJNA310114 |
| SRA |
SRP069018 |
Less...
More...