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| Status |
Public on May 25, 2017 |
| Title |
miR-seq to define mature miRNAs in human HEK293T cells that are targeted for degradation by the endonuclease Tudor-SN (i.e. by TumiD). |
| Organism |
Homo sapiens |
| Experiment type |
Non-coding RNA profiling by high throughput sequencing
|
| Summary |
We report that human Tudor-SN degrades particular miRNAs that contain CA or UA dinucleotides located more than five nucleotides from miRNA ends.
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| |
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| Overall design |
We defined miRNAs that are degraded by Tudor-SN by performing miR-seq on RNA isolated from HEK293T cells transiently transfected with Tudor-SN siRNA or, as a control, Control (Ctl) siRNA.
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| |
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| Contributor(s) |
Du P, Tian B, Elbarbary R, Miyoshi K, Maquat L, . Myers JR, Ashton J |
| Citation(s) |
28546213 |
| Submission date |
Feb 02, 2016 |
| Last update date |
May 15, 2019 |
| Contact name |
Lynne Maquat |
| Organization name |
University of Rochester
|
| Department |
Biochemistry and Biophysics
|
| Lab |
MC 3-8507C
|
| Street address |
601 Elmwood Ave
|
| City |
Rochester |
| State/province |
NY |
| ZIP/Postal code |
14642 |
| Country |
USA |
| |
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| Platforms (2) |
| GPL16791 |
Illumina HiSeq 2500 (Homo sapiens) |
| GPL18573 |
Illumina NextSeq 500 (Homo sapiens) |
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| Samples (12)
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| Relations |
| BioProject |
PRJNA310615 |
| SRA |
SRP069192 |
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