|
| Status |
Public on Sep 26, 2018 |
| Title |
Investigation of Bach2 function by regulating inflammatory response and heme-iron homeostasis of bone marrow macrophage. |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
|
| Summary |
Macrophages are central in regulating iron homeostasis. Transcription repressor Bach2 regulates by heme. Here we investigated the relationship between heme-regulated Bach2 and macrophage in bone marrow. We identified RFP-positive and negative macrophage were in bone marrow. We found that RFP-positive macrophage related with iron-heme homeostasis maintenance and RPF-negative population related with immune response. In RFP positive macrophage, we also found the lysosomal heme transporter hrg-1 was Bach2 direct target gene. Our results suggest that the function of the bone marrow macrophage alters according to expression of Bach2.
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| Overall design |
Gene expression in bone marrow macrophages cells of Bach2-td RFP reporter mice was measured. 3 independent mice ware used for each genotype.
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| Contributor(s) |
Watanabe-Matsui M, Igarashi K |
| Citation(s) |
30250186 |
| Submission date |
Mar 29, 2016 |
| Last update date |
Dec 27, 2018 |
| Contact name |
Kazuhiko Igarashi |
| E-mail(s) |
igarashi@med.tohoku.ac.jp
|
| Phone |
+81227177597
|
| Organization name |
Tohoku University
|
| Street address |
Seiryo 2-1
|
| City |
Sendai |
| ZIP/Postal code |
980-8575 |
| Country |
Japan |
| |
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| Platforms (1) |
| GPL13912 |
Agilent-028005 SurePrint G3 Mouse GE 8x60K Microarray (Feature Number version) |
|
| Samples (6)
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| GSM2101259 |
#58 bone marrow macrophage_RFP_negative |
| GSM2101260 |
#63 bone marrow macrophage_RFP_negative |
| GSM2101261 |
#66 bone marrow macrophage_RFP_negative |
| GSM2101262 |
#58 bone marrow macrophage_RFP_positive |
| GSM2101263 |
#63 bone marrow macrophage_RFP_positive |
| GSM2101264 |
#66 bone marrow macrophage_RFP_positive |
|
| Relations |
| BioProject |
PRJNA316726 |
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