|
| Status |
Public on May 01, 2016 |
| Title |
Epigenetic Profile in RBPJ Maintains Brain Tumor Initiating Cells through CDK9-mediated Transcriptional Elongation |
| Organism |
Homo sapiens |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
|
| Summary |
Glioblastomas display hierarchies with self-renewing cancer stem-like cells (CSCs). RNA sequencing and enhancer mapping revealed regulatory programs unique to CSCs causing upregulation of the iron transporter transferrin, the top differentially expressed gene compared to tissue-specific progenitors. Direct interrogation of iron uptake demonstrated CSCs potently extract iron from the microenvironment more effectively than other tumor cells. Systematic interrogation of iron flux determined that CSCs preferentially require transferrin receptor and ferritin - two core iron regulators - to propagate and form tumors in vivo. Depleting ferritin disrupted CSC mitotic progression, through the STAT3-FoxM1 regulatory axis, revealing an iron-regulated CSC pathway. Iron is a unique, primordial metal fundamental for earliest life forms, and on which CSCs have an epigenetically programmed, targetable dependence.
|
| |
|
| Overall design |
H3K27Ac histone modifications and RBPJ transcription factor binding were mapped by ChIP-seq in Glioblastoma initiating cells
|
| |
|
| Contributor(s) |
Xie Q, Liau BB, Miller TE, Bernstein BE, Rich JN |
| Citation(s) |
27322055 |
| Submission date |
Mar 30, 2016 |
| Last update date |
May 15, 2019 |
| Contact name |
Tyler E Miller |
| Organization name |
Cleveland Clinic Lerner Research Institute
|
| Department |
Stem Cell Biology and Regenerative Medicine
|
| Lab |
Jeremy Rich Lab
|
| Street address |
2111 E 96th Street, NE3-256
|
| City |
Cleveland |
| State/province |
Ohio |
| ZIP/Postal code |
44106 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL18573 |
Illumina NextSeq 500 (Homo sapiens) |
|
| Samples (6)
|
|
| This SubSeries is part of SuperSeries: |
| GSE79736 |
RBPJ Maintains Brain Tumor Initiating Cells through CDK9-mediated Transcriptional Elongation |
|
| Relations |
| BioProject |
PRJNA316862 |
| SRA |
SRP072572 |
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