|
| Status |
Public on Apr 15, 2016 |
| Title |
Aging-related virtually naïve memory T (TVNM) cells: a new stage in T cell response to persistent infections in humans |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
We conducted RNA-Seq on T cell subsets sorted from five donors to identify a transcriptional signature associated with virtually naïve memory T cells.
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| |
|
| Overall design |
We sorted various T cell subsets from five donors including TEMRAs, naïve T cells, effector memory T cells, central memory T cells, and virtually naïve memory T cells and used a template-switching-based library construction protocol to faciliate low-input RNA-Seq from tens to hundreds of thousands of cells in each subpopulation.
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| |
|
| Contributor(s) |
Sims PA |
| Citation(s) |
27270402 |
| Submission date |
Apr 14, 2016 |
| Last update date |
May 15, 2019 |
| Contact name |
Peter A Sims |
| E-mail(s) |
pas2182@columbia.edu
|
| Organization name |
Columbia University
|
| Street address |
3960 Broadway, Lasker 203AC
|
| City |
New York |
| State/province |
NY |
| ZIP/Postal code |
10032 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL18573 |
Illumina NextSeq 500 (Homo sapiens) |
|
| Samples (21)
|
|
| Relations |
| BioProject |
PRJNA318527 |
| SRA |
SRP073331 |
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