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| Status |
Public on Feb 13, 2018 |
| Title |
Evaluation of the immunogenicity of live-attenuated influenza vaccines in nasal epithelial cells in primary differentiated human nasal epithelial cells [RNA-Seq] |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
The host innate immune response to influenza virus is a key determinant of pathogenic outcomes and long-term protective responses against subsequent exposures. Comparison of the transcriptional profiles obtained 24 and 36 hrs post-infection showed that the magnitude of gene expression was greater in LAIV infected cells relative to that observed in WT infected cells. Functional enrichment analysis revealed that the antiviral and inflammatory responses was largely driven by type III IFN induction in both WT and LAIV infected cells. However, the enrichment of biological pathways involved in the recruitment of mononuclear leukocytes, antigen presenting cells, and T lymphocytes was uniquely observed in LAIV infected cells. These findings indicate that cell-intrinsic type III IFN-mediated innate immune responses in the nasal epithelium are not only crucial for viral clearance and attenuation, but may also play an important role in the immunogenicity of live-attenuated vaccines
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| Overall design |
Differentiated hNECs were infected at a multiplicity of infection (MOI) of 5 50% tissue culture infectious dose per cell. Prior to infection, cells were apically washed and the basolateral media was refreshed with 500 μl of fresh LHC Basal Medium. The virus inoculum was added to the apical compartment and incubated for 2 hours, after which the inoculum was aspirated and the apical chamber was washed three times. The plates were then returned to the incubator. RNA was isolated from Trizol homogenates from samples harvested at 24hpi and 36hpi. Gene expression microarray analysis was conducted on these samples.
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| Contributor(s) |
Forero A, Fenstermacher K, Wohlgemuth N, Carter V, Smith E, Nishida A, Peng X, Hayes M, Francis D, Treanor J, Morrison J, Klein SL, Lane A, Katze M, Pekosz A |
| Citation(s) |
28967519 |
| Submission date |
May 26, 2016 |
| Last update date |
May 15, 2019 |
| Contact name |
Michael Katze |
| E-mail(s) |
data@viromics.washington.edu
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| Organization name |
University of Washington
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| Department |
Microbiology
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| Lab |
Michael G. Katze, Ph.D
|
| Street address |
Rosen Building 960 Republican St.
|
| City |
Seattle |
| State/province |
WA |
| ZIP/Postal code |
98109-4325 |
| Country |
USA |
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| Platforms (1) |
| GPL18573 |
Illumina NextSeq 500 (Homo sapiens) |
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| Samples (6)
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| This SubSeries is part of SuperSeries: |
| GSE83285 |
Evaluation of the immunogenicity of live-attenuated influenza vaccines in nasal epithelial cells in primary differentiated human nasal epithelial cells |
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| Relations |
| BioProject |
PRJNA323488 |
| SRA |
SRP075770 |
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