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| Status |
Public on Aug 09, 2017 |
| Title |
Innate to adaptive: Human IFN-gamma producing CD4+ T cells can derive directly from CXCL8-producing recent thymic emigrants. |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
An unanticipated feature of the human neonatal CD4 T cell response is a robust capacity to produce CXCL8. However, this 'innate-like' function dissipates with age and is scarce in the adult. Here, we investigated the fate of CD4+CXCL8+ cells and their transition into conventional adaptive T cells. We show that CXCL8 is imprinted on immature thymocytes prior to TCR signalling and is maintained in T cell committed thymic progenitors and recent thymic emigrants (RTEs) of adults as well as neonates. Hence, rather than being unique to neonates, CXCL8-producing CD4+ T cells decrease with age in humans (and in humanised mice) owing to the decline in thymic output, coupled with the cells’ peripheral expansion. By cloning of CXCL8+CD4+ cells from cord blood, we were able to track effector function within daughter cells and demonstrate that these cells can convert to IFN-g producing cells. In sum, we provide direct evidence that ‘innate like’ CXCL8-producing CD4+ T cells emerge from the thymus and can transition into conventional adaptive Th1 cells
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| Overall design |
Examination of RNA-Seq count data from 96 single cells
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| Contributor(s) |
Das A, Rouault-Pierre K, Gomez-Tourino I, Wood K, Donaldson IM, Mein CA, Bonnet D, Hayday AC, Gibbons DL |
| Citation(s) |
28754679 |
| Submission date |
Jul 21, 2016 |
| Last update date |
May 15, 2019 |
| Contact name |
Deena L. Gibbons |
| E-mail(s) |
deena.gibbons@kcl.ac.uk
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| Phone |
+44 020 7188 0150
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| Organization name |
Kings College London
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| Department |
Dept. Immunology
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| Street address |
Great Maze Pond
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| City |
London |
| ZIP/Postal code |
SE1 9RT |
| Country |
United Kingdom |
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| Platforms (1) |
| GPL18573 |
Illumina NextSeq 500 (Homo sapiens) |
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| Samples (96)
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| Relations |
| BioProject |
PRJNA330840 |
| SRA |
SRP079213 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE84686_consolidated_raw_counts_final.txt.gz |
625.5 Kb |
(ftp)(http) |
TXT |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data are available on Series record |
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