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| Status |
Public on May 17, 2017 |
| Title |
inDrop single cell RNA-seq of hematopoietic cells derived from human pluripotent stem cells |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
We performed morphogen-directed differentiation of human PSCs into HE followed by combinatorial screening of 26 candidate HSC-specifying TFs for the potential to promote hematopoietic engraftment in irradiated immune deficient murine hosts. We recovered seven TFs (ERG, HOXA5, HOXA9, HOXA10, LCOR, RUNX1, SPI1) that together were sufficient to convert HE into hematopoietic stem and progenitor cells (HSPCs) that engraft primary and secondary murine recipients
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| |
|
| Overall design |
Examination of expression pattern in hematopoietic cells.
|
| |
|
| Contributor(s) |
Sugimura R, Daley G |
| Citation(s) |
28514439 |
| Submission date |
Aug 02, 2016 |
| Last update date |
May 15, 2019 |
| Contact name |
Areum Han |
| E-mail(s) |
areum.han@childrens.harvard.edu
|
| Organization name |
Boston Children's Hospital
|
| Lab |
Daley lab
|
| Street address |
Karp Family Building, 7th Floor. 300 Longwood Ave.
|
| City |
Boston |
| State/province |
MA |
| ZIP/Postal code |
02115 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL18573 |
Illumina NextSeq 500 (Homo sapiens) |
|
| Samples (2) |
| GSM2257877 |
HSPC from hiPSC_RS1 (single-cell RNA-seq) |
| GSM2257878 |
HSPC from CB_RS2 (single-cell RNA-seq) |
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| This SubSeries is part of SuperSeries: |
| GSE85112 |
Hematopoietic Stem and Progenitor Cells from Human Pluripotent Stem Cells via Transcription Factor Conversion of Hemogenic Endothelium |
|
| Relations |
| BioProject |
PRJNA336240 |
| SRA |
SRP080883 |
