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| Status |
Public on Mar 09, 2017 |
| Title |
Analysis of genome-wide occupancy of menin, MLL1 and MLL2 in MCF-7 cells |
| Organism |
Homo sapiens |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
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| Summary |
We performed ChIP-seq using antibodies directed at menin, MLL1 and MLL2 in MCF-7 cells in order to assess the genome-wide presence of these proteins in MCF-7 cells.
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| Overall design |
MCF-7 cells were synchronized in phenol red-free medium containing 10% charcoal dextran-treated fetal bovine serum (CDT medium). Cells were treated with 10 nM estradiol (E2) or vehicle for 45 minutes. Menin, MLL1 and MLL2 ChIP-seq was performed after vehicle treatment. In addition, the menin cistrome was defined 45 min after treatment with E2.
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| Contributor(s) |
Dreijerink KM, Groner AC |
| Citation(s) |
28273452 |
| Submission date |
Aug 08, 2016 |
| Last update date |
May 15, 2019 |
| Contact name |
Koen M.A. Dreijerink |
| E-mail(s) |
koendreijerink@yahoo.com
|
| Organization name |
Dana-Farber Cancer Institute
|
| Department |
Medical Oncology
|
| Street address |
450 Brookline Avenue
|
| City |
Boston |
| State/province |
MA |
| ZIP/Postal code |
02215 |
| Country |
USA |
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| Platforms (1) |
| GPL18573 |
Illumina NextSeq 500 (Homo sapiens) |
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| Samples (7)
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| Relations |
| BioProject |
PRJNA338168 |
| SRA |
SRP081098 |
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