 |
 |
GEO help: Mouse over screen elements for information. |
|
| Status |
Public on Aug 22, 2017 |
| Title |
RNAseq from polysomal RNA harvested from HCT116, MCF7 and SJSA cell lines treated with DMSO and Nutlin |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
To determine effects of p53 activation on levels of RNA associated with polysomes, we performed RNA-seq analysis of colorectal carcinoma cell line HCT116, breast carcinoma line MCF7, and osteosarcoma line SJSA treated with MDM2 inhibitor Nutlin.
|
| |
|
| Overall design |
Polysomal RNA was extracted from HCT116, MCF7 and SJSA cells treated with Nutlin, polyA enriched and subjected to RNA-seq protocol.
|
| |
|
| Contributor(s) |
Zaccara S, Galbraith MD, Andrysik Z, Pandey A, Espinosa JM |
| Citation(s) |
28904012 |
| Submission date |
Aug 30, 2016 |
| Last update date |
May 15, 2019 |
| Contact name |
Ahwan Pandey |
| E-mail(s) |
ahwan.pandey@ucdenver.edu
|
| Organization name |
University of Colorado Anschutz Medical Center
|
| Department |
Pharmacology
|
| Lab |
Espinosa Lab
|
| Street address |
12800 E. 19th Ave.
|
| City |
Aurora |
| State/province |
CO |
| ZIP/Postal code |
80045 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL17303 |
Ion Torrent Proton (Homo sapiens) |
|
| Samples (12)
|
| GSM2298374 |
HCT116, polysomal RNA, DMSO treated, replicate 1 |
| GSM2298375 |
HCT116, polysomal RNA, DMSO treated, replicate 2 |
| GSM2298376 |
HCT116, polysomal RNA, Nutlin treated, replicate 1 |
| GSM2298377 |
HCT116, polysomal RNA, Nutlin treated, replicate 2 |
| GSM2298378 |
MCF7, polysomal RNA, DMSO treated, replicate 1 |
| GSM2298379 |
MCF7, polysomal RNA, DMSO treated, replicate 2 |
| GSM2298380 |
MCF7, polysomal RNA, Nutlin treated, replicate 1 |
| GSM2298381 |
MCF7, polysomal RNA, Nutlin treated, replicate 2 |
| GSM2298382 |
SJSA, polysomal RNA, DMSO treated, replicate 1 |
| GSM2298383 |
SJSA, polysomal RNA, DMSO treated, replicate 2 |
| GSM2298384 |
SJSA, polysomal RNA, Nutlin treated, replicate 1 |
| GSM2298385 |
SJSA, polysomal RNA, Nutlin treated, replicate 2 |
|
| This SubSeries is part of SuperSeries: |
| GSE86222 |
Identification of a core p53 transcriptional program with highly fractionated tumor suppressive activity |
|
| Relations |
| BioProject |
PRJNA340954 |
| SRA |
SRP083725 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE86219_HD_pol.bs1.forward.deeptools.normalized.bigwig |
66.9 Mb |
(ftp)(http) |
BIGWIG |
| GSE86219_HD_pol.bs1.reverse.deeptools.normalized.bigwig |
63.6 Mb |
(ftp)(http) |
BIGWIG |
| GSE86219_HN_pol.bs1.forward.deeptools.normalized.bigwig |
69.6 Mb |
(ftp)(http) |
BIGWIG |
| GSE86219_HN_pol.bs1.reverse.deeptools.normalized.bigwig |
66.5 Mb |
(ftp)(http) |
BIGWIG |
| GSE86219_HTSeq_intersection-nonempty_counts_polysomal_only.tab.gz |
468.4 Kb |
(ftp)(http) |
TAB |
| GSE86219_MD_pol.bs1.forward.deeptools.normalized.bigwig |
58.7 Mb |
(ftp)(http) |
BIGWIG |
| GSE86219_MD_pol.bs1.reverse.deeptools.normalized.bigwig |
56.1 Mb |
(ftp)(http) |
BIGWIG |
| GSE86219_MN_pol.bs1.forward.deeptools.normalized.bigwig |
71.1 Mb |
(ftp)(http) |
BIGWIG |
| GSE86219_MN_pol.bs1.reverse.deeptools.normalized.bigwig |
68.0 Mb |
(ftp)(http) |
BIGWIG |
| GSE86219_SD_pol.bs1.forward.deeptools.normalized.bigwig |
69.0 Mb |
(ftp)(http) |
BIGWIG |
| GSE86219_SD_pol.bs1.reverse.deeptools.normalized.bigwig |
65.8 Mb |
(ftp)(http) |
BIGWIG |
| GSE86219_SN_pol.bs1.forward.deeptools.normalized.bigwig |
63.0 Mb |
(ftp)(http) |
BIGWIG |
| GSE86219_SN_pol.bs1.reverse.deeptools.normalized.bigwig |
60.8 Mb |
(ftp)(http) |
BIGWIG |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data are available on Series record |
|
|
|
|
 |
Less...
More...