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| Status |
Public on Jun 12, 2018 |
| Title |
Sequencing-based analyses characterize a tumor suppressive role of mir-1271 repressed by DNA hypermethylation in gastric cancer |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
Non-coding RNA profiling by high throughput sequencing
Methylation profiling by high throughput sequencing
|
| Summary |
To identify tumor suppressive microRNAs repressed by DNA hypermethylation in gastric cancer (GC), we analyzed methylome and miRNome of EpCAM+/CD44+ GC cells. Among a set of microRNAs hypermethylated and downregulated in GC, mir-1271 was uncovered as a microRNA repressed by DNA hypermethylation in GC. Forced expression of mir-1271 significantly suppressed growth, migration, and invasion of GC cells both in vitro and in vivo. To identify target genes and cancer signaling pathways regulated by mir-1271, we examined differentially-expressed genes responsive to mir-1271 by performing RNA-sequencing.
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| |
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| Overall design |
2 paired normal and GC cancer cells miRNA-seq, 3 GC cell lines MBD-seq, miR-1271 mimic treated GC cells and control cells RNA-seq
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| |
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| Contributor(s) |
Lim B, Baek S, Kim J, Kim S, Kim YS, Kim M |
| Citation(s) |
29862663 |
| Submission date |
Oct 10, 2016 |
| Last update date |
Jul 25, 2021 |
| Contact name |
Jong Hwan Kim |
| E-mail(s) |
kkjjhhk@kribb.re.kr
|
| Phone |
0821093299557
|
| Organization name |
Korea Research Institute of Bioscience and Biotechnology
|
| Department |
Genome structure research center
|
| Street address |
125 Gwahak-ro, Yuseong-gu
|
| City |
Daejeon |
| ZIP/Postal code |
ASI|KR|KS015|DAEJEON |
| Country |
South Korea |
| |
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| Platforms (2) |
| GPL11154 |
Illumina HiSeq 2000 (Homo sapiens) |
| GPL18573 |
Illumina NextSeq 500 (Homo sapiens) |
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| Samples (19)
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| Relations |
| BioProject |
PRJNA347596 |
| SRA |
SRP091347 |
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