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Status |
Public on Oct 31, 2018 |
Title |
RNA-Seq from early time points in the kidney differentiation protocol |
Organism |
Homo sapiens |
Experiment type |
Expression profiling by high throughput sequencing
|
Summary |
The kidney differentiation protocol takes induced pluripotent stem cells through to kidney organoid via directed differentiation in approximately 25 days. Initially the cells are grown in a monolayer in a dish before being pelleted on day seven. We have performed RNA sequencing on three replicates taken at two points before cells are pelleted: day 0, when they are still iPSCs, and day 4 when cells have been exposed to growth factors and have started differentiating.
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Overall design |
Gene expression profiling of six samples across two time points (Day 0 and Day 4)
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Contributor(s) |
Phipson B |
Citation(s) |
30573816 |
Submission date |
Oct 19, 2016 |
Last update date |
May 15, 2019 |
Contact name |
Belinda Phipson |
Organization name |
WEHI
|
Department |
Bioinformatics
|
Lab |
Phipson
|
Street address |
1G Royal Parade
|
City |
Parkville |
State/province |
VIC |
ZIP/Postal code |
3052 |
Country |
Australia |
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Platforms (1) |
GPL18573 |
Illumina NextSeq 500 (Homo sapiens) |
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Samples (6)
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Relations |
BioProject |
PRJNA349167 |
SRA |
SRP091776 |