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| Status |
Public on Oct 20, 2017 |
| Title |
Post-transcriptional Gene Silencing Mediated by microRNAs is Controlled by Nucleoplasmic Sfpq [Agilent] |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
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| Summary |
There is a growing body of evidence about the presence and the activity of the miRISC in the nucleus of mammalian cells. Here, we show by quantitative proteomic analysis that Ago2 interacts with nucleoplasmic Sfpq in a RNA-dependent fashion. By HITS-CLIP and transcriptomic analyses, we demonstrated that Sfpq directly controls the miRNA targeting of a subset of crucial miRNA-target mRNAs when it binds locally. Sfpq modulates miRNA targeting in both nucleoplasm and cytoplasm, indicating a nucleoplasmic imprinting of Sfpq-target mRNAs that influence miRNA targeting in both cellular compartments. Mechanistically, Sfpq binds to a sizeable set of long 3’UTR forming long aggregates to optimize miRNA position/recruitment to selected binding sites, as we show for Lin28A mRNA. These results extend the miRNA-mediated post-transcriptional gene silencing into the nucleoplasm and indicate that an unique Sfpq-dependent post-transcriptional strategy for controlling both nuclear and cytoplasmic gene expression takes place in cells during physio-pathological events.
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| Overall design |
Agilent one-color microarray of P19 cells in control, let-7a overexpression, SFPQ knockdown, let-7a overexpression and SFPQ knockdown. Four replicates for each condition.
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| Contributor(s) |
Bottini S, Hamouda-Tekaya N, Mategot R, Zaragosi L, Audebert S, Grandjean V, Mauduit C, Benahmed M, Barbry P, Repetto E, Trabucchi M |
| Citation(s) |
29084942 |
| Submission date |
Oct 21, 2016 |
| Last update date |
Jan 24, 2019 |
| Contact name |
Silvia Bottini |
| E-mail(s) |
silvia.bottini@unice.fr
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| Organization name |
C3m
|
| Street address |
299, chemin de la ginestiere
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| City |
Nice |
| ZIP/Postal code |
06200 |
| Country |
France |
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| Platforms (1) |
| GPL13912 |
Agilent-028005 SurePrint G3 Mouse GE 8x60K Microarray (Feature Number version) |
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| Samples (16)
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| This SubSeries is part of SuperSeries: |
| GSE89033 |
Post-transcriptional Gene Silencing Mediated by microRNAs is Controlled by Nucleoplasmic Sfpq |
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| Relations |
| BioProject |
PRJNA349981 |
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