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| Status |
Public on Apr 16, 2020 |
| Title |
Transcriptome analysis after knocking down class III HD ZIP transcription factors in SAM |
| Organism |
Arabidopsis thaliana |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
We ubiqoutesly expressed mir165a (pUBQ>>mir165a) in ap1 cal background for RNA-Seq studies. Expression of this transgenes in ap1 cal background gives phenotype similar to their expression in wild-type (WT) backgrounds. We induced the transgenes for 8 hours or 16 hours. As a control we used pUBQ::GR-LHG4 transgeneic line. Q-PCR analysis on extracted RNA showed the successful down regulation of known target genes of class III HD ZIPs.
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| Overall design |
2 different transgenic lines (pUBQ>>mir165as, and pUBQ::GR-LHG4) were used in ap1cal background. 2 time points (8hr and 16hr) were used for each transgenic line. 3 biological replicates were used for each time point.
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| Contributor(s) |
Ram H, Sahadevan S, Heisler M |
| Citation(s) |
32294082 |
| Submission date |
Nov 22, 2016 |
| Last update date |
Apr 16, 2020 |
| Contact name |
Hasthi Ram |
| E-mail(s) |
hrmundel84@gmail.com
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| Organization name |
NABI (National Agri-Food Biotechnology Institute)
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| Department |
Agriculture biotechnology
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| Lab |
Hasthi Ram
|
| Street address |
Sector 81
|
| City |
Mohali |
| ZIP/Postal code |
140306 |
| Country |
India |
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| Platforms (1) |
| GPL13222 |
Illumina HiSeq 2000 (Arabidopsis thaliana) |
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| Samples (12)
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| Relations |
| BioProject |
PRJNA354623 |
| SRA |
SRP093767 |
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