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| Status |
Public on May 22, 2017 |
| Title |
Time series RNA profiling of endotheilal cells in response to atheroprone and atheroprotective flows |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
To profile shear stress-regulated endothelial transcriptomes, we performed RNA-seq with HUVECs subjected to different shear flow conditions, including atheroprotective pulsatile shear (PS, 12±4 dyn/cm2) and atheroprone oscillatory shear (OS, 0.5±4 dyn/cm2), or kept as static control (ST) for four time periods (1, 4, 12 and 24 hours)
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| Overall design |
HUVECs cultured on collagen I-coated slides were exposed to PS, OS or ST. RNA were extracted with TRIzol and sequenced on Illumina HiSeq platform.
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| Contributor(s) |
Huang T, Wang K, Quon S, Nguyen P, Chang T, Chen Z, Li Y, Subramanian S, Shyy J, Chien S |
| Citation(s) |
28500253 |
| Submission date |
Dec 16, 2016 |
| Last update date |
May 15, 2019 |
| Contact name |
Shu Chien |
| E-mail(s) |
shuchien@ucsd.edu
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| Organization name |
University of California, San Diego
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| Department |
Institute of Engineering In Medicine
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| Street address |
9500 Gilman Drive
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| City |
La Jolla |
| State/province |
CA |
| ZIP/Postal code |
92093 |
| Country |
USA |
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| Platforms (1) |
| GPL15433 |
Illumina HiSeq 1000 (Homo sapiens) |
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| Samples (24)
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| Relations |
| BioProject |
PRJNA357824 |
| SRA |
SRP095259 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE92506_DEseq2_wholeTable.txt.gz |
3.8 Mb |
(ftp)(http) |
TXT |
| GSE92506_RawCounts.txt.gz |
1.0 Mb |
(ftp)(http) |
TXT |
| GSE92506_VarStabTrf.txt.gz |
3.6 Mb |
(ftp)(http) |
TXT |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data are available on Series record |
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