|
| Status |
Public on Jan 17, 2013 |
| Title |
vif_6hrs_rep3 |
| Sample type |
RNA |
| |
|
| Source name |
Jurkat Trex expressing Vif_6hrs PMA/PHA
|
| Organism |
Homo sapiens |
| Characteristics |
cell line: Invitrogen Jurkat Trex (Invitrogen)
genotype/variation: expressing stably integrated, tetracycline inducible HIV-1 Vif
treated with: 50 ng/ml PMA and 1 ug/ul PHA for 6hrs
|
| Treatment protocol |
cells were treated with 50 ng/ml PMA and 1 ug/ul PHA for 4 or 6 hours as indicated
|
| Growth protocol |
cells were grown in RPMI-1640 for two days, then exposed to 1 ug/ml doxycycline for 16 hrs
|
| Extracted molecule |
total RNA |
| Extraction protocol |
TRIZOL was used according to the manufacturer's instructions
|
| Label |
biotin
|
| Label protocol |
total RNA was amplified by SPRI.
|
| |
|
| Hybridization protocol |
hybridizations were performed according to Affymetrix standard protocols.
|
| Scan protocol |
scans were performed according to Affymetrix standard protocols.
|
| Description |
SAMPLE 12
|
| Data processing |
data were analyzed using the MMBGX software platform.
expression level defined by MMBGX. Note that the summaries (average) of the replicates are calculated after performing a loess curve.
|
| |
|
| Submission date |
Nov 28, 2012 |
| Last update date |
Jan 17, 2013 |
| Contact name |
Nevan Krogan |
| E-mail(s) |
nevan.krogan@ucsf.edu
|
| Phone |
415-476-2980
|
| Fax |
415-514-9736
|
| Organization name |
University of California, San Francisco
|
| Department |
Cellular & Molecular Pharmacology
|
| Lab |
Krogan
|
| Street address |
1700 4th Street
|
| City |
San Francisco |
| ZIP/Postal code |
94158 |
| Country |
USA |
| |
|
| Platform ID |
GPL16332 |
| Series (2) |
| GSE42574 |
CBFb Stabilizes HIV Vif to Counteract APOBEC3 at the Expense of RUNX1 Target Gene Expression [gene expression] |
| GSE42576 |
CBFb Stabilizes HIV Vif to Counteract APOBEC3 at the Expense of RUNX1 Target Gene Expression |
|
