|
| Status |
Public on Jan 30, 2014 |
| Title |
14844 - Veh |
| Sample type |
RNA |
| |
|
| Source name |
Sorted GFP+ T-ALL from Vehicle treated mouse
|
| Organism |
Mus musculus |
| Characteristics |
cell line: 14844
cell type: Notch induced T-lymphoblastic leukemia (T-ALL)
genotype/variation: ZFX flox
|
| Treatment protocol |
Leukemia cells were treated with Tamoxifen as they expanded in murine hosts in-vivo. Tamoxifen was administered via 5 mg daily gavage for three days.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Used the Ambion WT labelling kit as per manufacturer's instructions using 200ng of starting total RNA
|
| |
|
| Hybridization protocol |
Biotinylated DNA probe was made according to the standard Affymetrix protocol
|
| Scan protocol |
Arrays were scanned according to the Affymetrix protocol
|
| Data processing |
Affymetrix Expression Console (1.1) was used for RMA normalization of all microarray data.
|
| |
|
| Submission date |
Dec 19, 2012 |
| Last update date |
Jan 30, 2014 |
| Contact name |
Stuart Weisberg |
| E-mail(s) |
spw13@cumc.columbia.edu
|
| Organization name |
Columbia University
|
| Department |
Pathology and Cell Biology
|
| Lab |
Weisberg
|
| Street address |
650 W 168th Street
|
| City |
New York |
| State/province |
NY |
| ZIP/Postal code |
10032 |
| Country |
USA |
| |
|
| Platform ID |
GPL6246 |
| Series (2) |
| GSE43020 |
Effect of genetic Zfx deletion on gene expression in Notch induced T-ALL |
| GSE43022 |
ZFX controls the propagation and cell-of-origin characteristics of acute leukemia |
|
