|
Status |
Public on Jan 30, 2014 |
Title |
14846 - Veh |
Sample type |
RNA |
|
|
Source name |
Sorted GFP+ T-ALL from Vehicle treated mouse
|
Organism |
Mus musculus |
Characteristics |
cell line: 14846 cell type: Notch induced T-lymphoblastic leukemia (T-ALL) genotype/variation: ZFX flox
|
Treatment protocol |
Leukemia cells were treated with Tamoxifen as they expanded in murine hosts in-vivo. Tamoxifen was administered via 5 mg daily gavage for three days.
|
Extracted molecule |
total RNA |
Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions.
|
Label |
biotin
|
Label protocol |
Used the Ambion WT labelling kit as per manufacturer's instructions using 200ng of starting total RNA
|
|
|
Hybridization protocol |
Biotinylated DNA probe was made according to the standard Affymetrix protocol
|
Scan protocol |
Arrays were scanned according to the Affymetrix protocol
|
Data processing |
Affymetrix Expression Console (1.1) was used for RMA normalization of all microarray data.
|
|
|
Submission date |
Dec 19, 2012 |
Last update date |
Jan 30, 2014 |
Contact name |
Stuart Weisberg |
E-mail(s) |
spw13@cumc.columbia.edu
|
Organization name |
Columbia University
|
Department |
Pathology and Cell Biology
|
Lab |
Weisberg
|
Street address |
650 W 168th Street
|
City |
New York |
State/province |
NY |
ZIP/Postal code |
10032 |
Country |
USA |
|
|
Platform ID |
GPL6246 |
Series (2) |
GSE43020 |
Effect of genetic Zfx deletion on gene expression in Notch induced T-ALL |
GSE43022 |
ZFX controls the propagation and cell-of-origin characteristics of acute leukemia |
|