|
| Status |
Public on Mar 01, 2013 |
| Title |
SFRS2_MEF_rep4_tetOFF |
| Sample type |
RNA |
| |
|
| Source name |
MEF cells from tet-repressible SFRS2-HA mice, treated with doxycycline
|
| Organism |
Mus musculus |
| Characteristics |
transgene: tet-repressible SFRS2-HA
cell type: MEF
treatment: tet off
genetic background: C57BL/6
|
| Treatment protocol |
4 biological replicate sets of SFRS1 or SFRS2 tet-repressible MEFs were grown for 5 days in DMEM -/+ 10ug/ml docycline.
|
| Growth protocol |
4 biological replicate sets of SFRS1 or SFRS2 tet-repressible MEFs were grown for 5 days in DMEM -/+ 10ug/ml docycline.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was isolated with the RNAeasy kid (Qiagen), then treated with Ribominus (Invitrogen).
|
| Label |
biotin
|
| Label protocol |
cDNA synthesis and amplification was performed using the GeneChip® Whole Transcript Sense Target Labeling and Control Reagents kit (Affymetrix). Targets were enzymatically fragmented and biotinylated using the WT Target Labeling Kit (Affymetrix)
|
| |
|
| Hybridization protocol |
Hybridization was performed as specified, with the GeneChip Hybridization Wash and Stain Kit (Affymetrix)
|
| Scan protocol |
Affymetrix Gene ChIP Scanner 3000 7G
|
| Data processing |
Affymetrix package (Affy Power Tools) apt-probeset-summarize. Iter-plier algorithm used to quantify probesets.
GPL13185_mjay.pgf
GPL13185_mjay.clf
|
| |
|
| Submission date |
Feb 22, 2013 |
| Last update date |
Mar 01, 2013 |
| Contact name |
Lily Shiue |
| E-mail(s) |
lshiue@ucsc.edu
|
| Phone |
831-459-2612
|
| Organization name |
UCSC
|
| Department |
MCD Biology
|
| Lab |
Ares
|
| Street address |
1156 High Street
|
| City |
Santa Cruz |
| State/province |
CA |
| ZIP/Postal code |
95064 |
| Country |
USA |
| |
|
| Platform ID |
GPL13185 |
| Series (1) |
| GSE44583 |
Genome-wide Analysis Reveals SR Protein Cooperation and Competition in Regulated Splicing |
|
