|
Status |
Public on Jul 20, 2013 |
Title |
Heart failure ISACHC class 3 Patient 8 |
Sample type |
RNA |
|
|
Channel 1 |
Source name |
whole blood
|
Organism |
Canis lupus familiaris |
Characteristics |
breed: Dachshund tissue: whole blood drawn from the cephalic or jugular veins and collected into Rneasy Protect Animal Blood Tubes (Qiagen, USA) disease state: Heart failure ISACHC class 3
|
Treatment protocol |
Blood collection into Rneasy Protect Animal Blood Tubes (Qiagen, USA).
|
Extracted molecule |
total RNA |
Extraction protocol |
Total RNA extracted using Rneasy Protect Animal Blood Kit following manufacturer's instructions (Qiagen, USA)
|
Label |
Cy5
|
Label protocol |
according to the manufacturer's protocol
|
|
|
Channel 2 |
Source name |
common reference sample compsed of total RNAs isolated from whole blood from 10 healthy dogs
|
Organism |
Canis lupus familiaris |
Characteristics |
tissue: whole blood drawn from the cephalic or jugular veins and collected into Rneasy Protect Animal Blood Tubes (Qiagen, USA) disease state: healthy
|
Treatment protocol |
Blood collection into Rneasy Protect Animal Blood Tubes (Qiagen, USA).
|
Extracted molecule |
total RNA |
Extraction protocol |
Total RNA extracted using Rneasy Protect Animal Blood Kit following manufacturer's instructions (Qiagen, USA)
|
Label |
Cy3
|
Label protocol |
according to the manufacturer's protocol
|
|
|
|
Hybridization protocol |
according to the manufacturer's protocol
|
Scan protocol |
Scanned on an Agilent G2505C US10353831 scanner. Images were quantified using Agilent Feature Extraction Software (version 10.7.3.1).
|
Data processing |
Agilent Feature Extraction Software (v 10.7.3.1) was used for background subtraction and LOWESS normalization.
|
|
|
Submission date |
Jun 26, 2013 |
Last update date |
Sep 07, 2013 |
Contact name |
Magdalena Łój |
E-mail(s) |
magda.loj@gmail.com
|
Organization name |
Warsaw University of Life Sciences
|
Department |
Department of Physiological Sciences
|
Street address |
Nowoursynowska 166
|
City |
Warsaw |
ZIP/Postal code |
02-787 |
Country |
Poland |
|
|
Platform ID |
GPL15379 |
Series (1) |
GSE48319 |
The transcriptomic profile of peripheral blood nuclear cells in dogs with heart failure |
|