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Status |
Public on Jun 22, 2014 |
Title |
PMA_mock |
Sample type |
RNA |
|
|
Source name |
MCF7, mock siRNA, PMA
|
Organism |
Homo sapiens |
Characteristics |
cell line: MCF7 treatment: mock siRNA (48h) and PMA(0.65ng/ml) for 60h
|
Treatment protocol |
Cells were treated with mock or PKC siRNA (20nM) for 48h and then left or stimulated with PMA (0.65ng/ml) for 60h.
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Growth protocol |
Cells were cultured at 37C in low glucose DMEM (Gibco) and supplemented with 10% FCS, 2mM L-glutamine, and 0.1% PSN antibiotics.
|
Extracted molecule |
total RNA |
Extraction protocol |
Trizol was used to extract RNA as per manufacturers instructions.
|
Label |
biotin
|
Label protocol |
Samples were labelled as per Affymetrix standard protocols
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|
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Hybridization protocol |
Samples were hybridised as per Affymetrix standard protocols
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Scan protocol |
Samples were scanned as per Affymetrix standard protocols
|
Description |
MCF7 cells treated with mock siRNA (48h) and PMA (60h)
|
Data processing |
Arrays were RMA normalized using Affymetrix Powertools and only ‘main’ and ‘rescue’ probesets were used for analysis. Differences of Log2 0.5 (1.4-fold) were used as cut-off for expression changes.
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Submission date |
Dec 12, 2013 |
Last update date |
Jun 22, 2014 |
Contact name |
Kristine Hardy |
E-mail(s) |
kristine.hardy@anu.edu.au
|
Organization name |
University of Canberra
|
Lab |
Cytokine Gene Expression
|
Street address |
University of Canberra
|
City |
Bruce |
State/province |
ACT |
ZIP/Postal code |
0200 |
Country |
Australia |
|
|
Platform ID |
GPL16686 |
Series (2) |
GSE53267 |
The role of PKCtheta in Epithelial to Mesenchymal Transistion |
GSE53335 |
Regulation of inducible genes in epithelial to mesenchymal transition by chromatinized PKC-theta |
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