|
| Status |
Public on Jun 22, 2014 |
| Title |
The role of PKCtheta in Epithelial to Mesenchymal Transistion |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
|
| Summary |
Epithelial to mesenchymal transition (EMT) is activated during cancer invasion and metastasis, enriches for cancer stem cells (CSCs), and contributes to therapeutic resistance and disease recurrence. Signal transduction kinases play a pivotal role as chromatin-anchored proteins in eukaryotes. Here we report for the first time that protein kinase C-theta (PKC-θ) regulates EMT by acting as a critical chromatin-anchored switch for inducible genes. Genome-wide transcriptome analysis identifies a unique cohort of inducible PKC-θ-sensitive genes in MCF7 cells stimulated with phorbol ester.
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| |
|
| Overall design |
MCF7 Cells treated with mock or siRNA against PKCtheta were left unstimulated or stimulated with PMA. No replicates.
|
| |
|
| Contributor(s) |
Hardy K, Zafar A, Wu F, Rao S |
| Citation(s) |
24891615 |
| Submission date |
Dec 12, 2013 |
| Last update date |
Mar 15, 2019 |
| Contact name |
Kristine Hardy |
| E-mail(s) |
kristine.hardy@anu.edu.au
|
| Organization name |
University of Canberra
|
| Lab |
Cytokine Gene Expression
|
| Street address |
University of Canberra
|
| City |
Bruce |
| State/province |
ACT |
| ZIP/Postal code |
0200 |
| Country |
Australia |
| |
|
| Platforms (1) |
| GPL16686 |
[HuGene-2_0-st] Affymetrix Human Gene 2.0 ST Array [transcript (gene) version] |
|
| Samples (4)
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| This SubSeries is part of SuperSeries: |
| GSE53335 |
Regulation of inducible genes in epithelial to mesenchymal transition by chromatinized PKC-theta |
|
| Relations |
| BioProject |
PRJNA231741 |
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