|
| Status |
Public on Jun 22, 2014 |
| Title |
nonstim_PKCsiRNA |
| Sample type |
RNA |
| |
|
| Source name |
MCF7, PKCtheta siRNA
|
| Organism |
Homo sapiens |
| Characteristics |
cell line: MCF7
treatment: PKCtheta siRNA (48h)
|
| Treatment protocol |
Cells were treated with mock or PKC siRNA (20nM) for 48h and then left or stimulated with PMA (0.65ng/ml) for 60h.
|
| Growth protocol |
Cells were cultured at 37C in low glucose DMEM (Gibco) and supplemented with 10% FCS, 2mM L-glutamine, and 0.1% PSN antibiotics.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol was used to extract RNA as per manufacturers instructions.
|
| Label |
biotin
|
| Label protocol |
Samples were labelled as per Affymetrix standard protocols
|
| |
|
| Hybridization protocol |
Samples were hybridised as per Affymetrix standard protocols
|
| Scan protocol |
Samples were scanned as per Affymetrix standard protocols
|
| Description |
MCF7 cells treated with PKCtheta siRNA (48h)
|
| Data processing |
Arrays were RMA normalized using Affymetrix Powertools and only ‘main’ and ‘rescue’ probesets were used for analysis. Differences of Log2 0.5 (1.4-fold) were used as cut-off for expression changes.
|
| |
|
| Submission date |
Dec 12, 2013 |
| Last update date |
Jun 22, 2014 |
| Contact name |
Kristine Hardy |
| E-mail(s) |
kristine.hardy@anu.edu.au
|
| Organization name |
University of Canberra
|
| Lab |
Cytokine Gene Expression
|
| Street address |
University of Canberra
|
| City |
Bruce |
| State/province |
ACT |
| ZIP/Postal code |
0200 |
| Country |
Australia |
| |
|
| Platform ID |
GPL16686 |
| Series (2) |
| GSE53267 |
The role of PKCtheta in Epithelial to Mesenchymal Transistion |
| GSE53335 |
Regulation of inducible genes in epithelial to mesenchymal transition by chromatinized PKC-theta |
|
