|
| Status |
Public on Mar 28, 2016 |
| Title |
LOXL2 delta 72 |
| Sample type |
RNA |
| |
|
| Source name |
KYSE150 cells expressing LOXL2 delta72
|
| Organism |
Homo sapiens |
| Characteristics |
cell line: human esophageal squamous carcinoma cell line KYSE150
genotype/variation: LOXL2 delta72
|
| Treatment protocol |
KYSE 150 cells were transfected with vector, pcDNA3.1 LOXL2 WT or pcDNA3.1 LOXL2 delta72, respectively. After 48 hours transfection, RNA extractions were carried out, and the expressions of LOXL2 were examined by RT-PCR. The samples were sent to perfor hybridization on Affymetrix microarrays.
|
| Growth protocol |
cell lines were maintained in RPMI 1640 medium (Invitrogen) containing 10% fetal calf serum (FCS)at 5% CO2 at 37℃
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 100 ng total RNA (GeneChip 3'IVT Express Kit User Manual, 2008, Affymetrix).
|
| |
|
| Hybridization protocol |
Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
|
| Scan protocol |
GeneChips were scanned using the Affymetrix® GeneChip® Command Console® (AGCC) version 3.0
|
| Description |
Gene expression data from KYSE150 cells expressing LOXL2 delta72
|
| Data processing |
The data were analyzed with Partek GS 6.5 using Affymetrix Expression Console and global scaling as normalization method.
|
| |
|
| Submission date |
Dec 24, 2013 |
| Last update date |
Mar 28, 2016 |
| Contact name |
Haiying Zou |
| E-mail(s) |
zouhaiying73@163.com
|
| Organization name |
Shantou University Medical College
|
| Department |
Department of Biochemistry and Molecular Biology
|
| Street address |
22 Xinling Road
|
| City |
Shantou |
| State/province |
Guangdong |
| ZIP/Postal code |
515041 |
| Country |
China |
| |
|
| Platform ID |
GPL15207 |
| Series (1) |
| GSE53645 |
Expression data in human esophageal squamous carcinoma cell line KYSE150 with LOXL2 delta 72 over-expression |
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