|
| Status |
Public on Dec 15, 2014 |
| Title |
Fetal kidney |
| Sample type |
RNA |
| |
|
| Source name |
kidney
|
| Organism |
Homo sapiens |
| Characteristics |
subject status: control
developmental stage: embryo
sample type: normal fetal kidney
tissue: kidney
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted from each of the four kidney samples: the index patient's kidney, a normal age-matched kidney, a normal fetal kidney and a normal adult kidney using TRIZOL .
|
| Label |
biotin
|
| Label protocol |
RNA samples were used to prepare biotinylated target DNA, according to manufacturer’s recommendations. The target complementary DNA (cDNA) generated from each sample was processed as per manufacturer’s recommendation using an Affymetrix GeneChip Instrument System
|
| |
|
| Hybridization protocol |
www.affymetrix.com/user/login.jsp?toURL=/support/file_download.affx?onloadforward=/support/downloads/manuals/3_ivt_express_kit_manual.pdf
|
| Scan protocol |
www.affymetrix.com/user/login.jsp?toURL=/support/file_download.affx?onloadforward=/support/downloads/manuals/3_ivt_express_kit_manual.pdf
|
| Description |
D178_AV04_FK__LIN
|
| Data processing |
The analysis was performed with RMA algorithm in PARTEK Genomic suite 6.6. The data was processed further for fold-change calculation.
|
| |
|
| Submission date |
Jan 20, 2014 |
| Last update date |
Oct 25, 2022 |
| Contact name |
jasmine Jacob |
| E-mail(s) |
j-jacob@sheba.health.gov.il
|
| Phone |
0523790500
|
| Organization name |
Sheba Medical Center at Tel HaShomer
|
| Street address |
haChevel 33
|
| City |
Shoham |
| State/province |
-Select- |
| ZIP/Postal code |
60850 |
| Country |
Israel |
| |
|
| Platform ID |
GPL15207 |
| Series (1) |
| GSE54227 |
A human integrin-α3 mutation confers major renal developmental defects. |
|
