|
| Status |
Public on Apr 01, 2015 |
| Title |
hypoxia serum plus |
| Sample type |
RNA |
| |
|
| Source name |
hypoxia serum plus
|
| Organism |
Homo sapiens |
| Characteristics |
cell line: OVSAYO
cell type: human ovarian cancer cells
culture condition: hypoxia serum plus
culture duration: 16 hours
|
| Growth protocol |
An ovarian cancer cell line, OVSAYO was cultured under four culture conditions (normoxia and fetal calf serum (FCS) plus, normoxia and FCS minus, hypoxia and FCS plus, and hypoxia and FCS minus conditions) in RPMI1640 medium for 16 hours.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was isolated using SV total RNA isolation kit (Promega, Madison, WI, USA) according to recommended standard protocol.
|
| Label |
Cy3
|
| Label protocol |
Labeling (Cy3) was performed using NimbleGen one-colour DNA labeling kit (Roche, Indianapolis, IN, USA) according to recommended standard operating protocol provided with gene expression microarray.
|
| |
|
| Hybridization protocol |
Hybridization to 2006-08-03_HG18_60mer_expr array (Euk expr 385K catalog Arr Del (NimbleGen, Roche, Madison, WI, USA)) was performed using NimbleGen Hybridization kit (Roche, Madison, WI, USA) according to recommended standard operating protocol provided with gene expression microarray.
|
| Scan protocol |
Scanning was performed by NimbleScan v2.6 software using GenePix Personal 4100A instrument (Axon Instruments, Sunyvale, CA, USA).
|
| Description |
OVSAYO cells were cultured under 1%O2 with 10%FCS for 16h.
|
| Data processing |
Scanned TIFF images were processed by NimbleScan v2.6 software (Roche NimbleGen, Inc.) to obtain raw data (pair) files. Data were further processed by same software to obtain normalized data (norm_RMA.pair and RMA.Calls files). These data were analyzed by contract research service at Subio Inc. (Kagoshima, Japan, www.subio.jp/) using Subio platform v 1.14 software.
|
| |
|
| Submission date |
Mar 04, 2014 |
| Last update date |
Apr 01, 2015 |
| Contact name |
Shiro Koizume |
| E-mail(s) |
skoizume@gancen.asahi.yokohama.jp
|
| Organization name |
Kanagawa Cancer Center Research Institute
|
| Street address |
2-3-2 Nakao Asahi-ku
|
| City |
Yokohama |
| State/province |
Please Select |
| ZIP/Postal code |
241-8515 |
| Country |
Japan |
| |
|
| Platform ID |
GPL15143 |
| Series (2) |
| GSE55564 |
Identification of genes activated in cancer cells cultured under hypoxia and serum-free condition |
| GSE55565 |
Identification of genes activated under CoCl2 treatment in an Sp1 dependent manner and under hypoxia without serum condition in an ovarian cancer cell line |
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