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Sample GSM1674098 Query DataSets for GSM1674098
Status Public on Nov 09, 2015
Title WHIM12 siCNTL Vehicle Treated Replicate1
Sample type RNA
 
Source name WHIM12 cells transfected with CNTL siRNA
Organism Homo sapiens
Characteristics tumor-derived cell line: WHIM12
transfection: CNTL siRNA
treatment: vehicle
Treatment protocol SUM159 and WHIM12 cells were reverse transfected with Dharmacon SMART siRNA pools in 6 well dishes. For SUM159 cells, 60 hours post transfection, cells were treated with either vehicle or 10ng/mL TGFB for 3 hours. For WHIM12 cells, 48 hours post transfection, cells were treated with either vehicle or 10ng/mL TGFB for 3 hours.
Growth protocol SUM159 and WHIM12 cells were grown in the F12, (Gibco) and MEM (Gibco), respectively, and split 1:3 every two days.
Extracted molecule total RNA
Extraction protocol Total RNA was isolated using Sigma GenElute RNA Isolation Kit
Label Biotin
Label protocol The WT Expression HT Kit for Robotics (Ambion) was used to generate sense-strand cDNA from total RNA. Following synthesis of sense-strand cDNA, the cDNA was fragmented and labeled with the Affymetrix GeneChip HT Terminal Labeling Kit.
 
Hybridization protocol Fragmented and labeled cDNA was used to prepare a hybridization cocktail with the Affymetrix GeneTitan Hybridization Wash and Stain Kit for WT Arrays.
Scan protocol Hybridization, washing, staining and scanning of the Affymtrix peg plate arrays was carried out using the Affymetrin GeneTitan MC Instrument.
Description Gene expression data from control transfected and vehicle treated WHIM12 cells
Data processing Affymetrix Expression Console Software was used for basic data analysis and quality control (RMA normalization).
 
Submission date May 04, 2015
Last update date Nov 10, 2015
Contact name Angelique Whitehurst
E-mail(s) angelique.whitehurst@utsouthwestern.edu
Phone 214-645-6066
Organization name UT Southwestern
Department Simmons Cancer Center
Street address 5323 Harry Hines Blvd.
City Dallas
State/province TX
ZIP/Postal code 75390-8807
Country USA
 
Platform ID GPL11532
Series (2)
GSE63984 Expression data to identify putative transcriptional targets of ZNF165 in Triple Negative Breast Cancer (TNBC)
GSE63986 Integrative Functional Characterization of Cancer-Testis Antigens Defines Obligate Participation in Multiple Hallmarks of Cancer

Data table header descriptions
ID_REF
VALUE Linear GC-RMA Signal

Data table
ID_REF VALUE
7892501 47.93127
7892502 12.46701
7892503 4.791291
7892504 144.6888
7892505 5.611936
7892506 27.81034
7892507 46.93134
7892508 26.1802
7892509 1480.853
7892510 54.45269
7892511 13.18799
7892512 22.35042
7892513 16.5217
7892514 1721.496
7892515 169.8104
7892516 35.91548
7892517 59.64443
7892518 14.53233
7892519 15.35998
7892520 170.1666

Total number of rows: 33297

Table truncated, full table size 549 Kbytes.




Supplementary file Size Download File type/resource
GSM1674098_12_siCNTL_rep1.CEL.gz 4.2 Mb (ftp)(http) CEL
Processed data included within Sample table

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