|
| Status |
Public on Nov 09, 2015 |
| Title |
Expression data to identify putative transcriptional targets of ZNF165 in Triple Negative Breast Cancer (TNBC) |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
|
| Summary |
We found that the cancer testis antigen, ZNF165, is required for viability and can modulate TGFβ-induced gene expression in mesenchymal, Claudin-Low, TNBC. We employed the Affymetrix microarray platform to uncover transcriptionally modulated genes following ZNF165 depletion and TGFβ stimulation using the Claudin-low TNBC tumor-derived cell lines, SUM159 as a model. Our results provide insight into how ZNF165 globally modulates TGFβ signaling.
We used microarray analysis to uncover the transcriptional network controlled by ZNF165 and clarify the role of ZNF165 in global TGFβ signaling
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| |
|
| Overall design |
SUM159 and WHIM12 cells were reverse transfected with either non-targeting or ZNF165 siRNA. For SUM159 cells, sixty hours post-transfection, cells were exposed to TGFβ for 3 hours. For WHIM12, forty eight hours post-transfection, cells were exposed to TGFβ for 3 hours. Total RNA was isolated and hybridized on to Affymetrix microarrays. Each experiment was repeated three times.
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| |
|
| Contributor(s) |
Whitehurst AW, Maxfield KE |
| Citation(s) |
26567849 |
| Submission date |
Dec 09, 2014 |
| Last update date |
Nov 08, 2016 |
| Contact name |
Angelique Whitehurst |
| E-mail(s) |
angelique.whitehurst@utsouthwestern.edu
|
| Phone |
214-645-6066
|
| Organization name |
UT Southwestern
|
| Department |
Simmons Cancer Center
|
| Street address |
5323 Harry Hines Blvd.
|
| City |
Dallas |
| State/province |
TX |
| ZIP/Postal code |
75390-8807 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL11532 |
[HuGene-1_1-st] Affymetrix Human Gene 1.1 ST Array [transcript (gene) version] |
|
| Samples (24)
|
| GSM1561805 |
SUM159 siCNTL Vehicle Treated Replicate1 |
| GSM1561806 |
SUM159 siCNTL TGFB Treated Replicate1 |
| GSM1561807 |
SUM159 siZNF165 Vehicle Treated Replicate1 |
| GSM1561808 |
SUM159 siZNF165 TGFB Treated Replicate1 |
| GSM1561809 |
SUM159 siCNTL Vehicle Treated Replicate2 |
| GSM1561810 |
SUM159 siCNTL TGFB Treated Replicate2 |
| GSM1561811 |
SUM159 siZNF165 Vehicle Treated Replicate2 |
| GSM1561812 |
SUM159 siZNF165 TGFB Treated Replicate2 |
| GSM1561813 |
SUM159 siCNTL Vehicle Treated Replicate3 |
| GSM1561814 |
SUM159 siCNTL TGFB Treated Replicate3 |
| GSM1561815 |
SUM159 siZNF165 Vehicle Treated Replicate3 |
| GSM1561816 |
SUM159 siZNF165 TGFB Treated Replicate3 |
| GSM1674098 |
WHIM12 siCNTL Vehicle Treated Replicate1 |
| GSM1674099 |
WHIM12 siCNTL TGFB Treated Replicate1 |
| GSM1674100 |
WHIM12 siZNF165 Vehicle Treated Replicate1 |
| GSM1674101 |
WHIM12 siZNF165 TGFB Treated Replicate1 |
| GSM1674102 |
WHIM12 siCNTL Vehicle Treated Replicate2 |
| GSM1674103 |
WHIM12 siCNTL TGFB Treated Replicate2 |
| GSM1674104 |
WHIM12 siZNF165 Vehicle Treated Replicate2 |
| GSM1674105 |
WHIM12 siZNF165 TGFB Treated Replicate2 |
| GSM1674106 |
WHIM12 siCNTL Vehicle Treated Replicate3 |
| GSM1674107 |
WHIM12 siCNTL TGFB Treated Replicate3 |
| GSM1674108 |
WHIM12 siZNF165 Vehicle Treated Replicate3 |
| GSM1674109 |
WHIM12 siZNF165 TGFB Treated Replicate3 |
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| This SubSeries is part of SuperSeries: |
| GSE63986 |
Integrative Functional Characterization of Cancer-Testis Antigens Defines Obligate Participation in Multiple Hallmarks of Cancer |
|
| Relations |
| BioProject |
PRJNA269723 |
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