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Status |
Public on Nov 09, 2015 |
Title |
WHIM12 siCNTL Vehicle Treated Replicate3 |
Sample type |
RNA |
|
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Source name |
WHIM12 cells transfected with CNTL siRNA
|
Organism |
Homo sapiens |
Characteristics |
tumor-derived cell line: WHIM12 transfection: CNTL siRNA treatment: vehicle
|
Treatment protocol |
SUM159 and WHIM12 cells were reverse transfected with Dharmacon SMART siRNA pools in 6 well dishes. For SUM159 cells, 60 hours post transfection, cells were treated with either vehicle or 10ng/mL TGFB for 3 hours. For WHIM12 cells, 48 hours post transfection, cells were treated with either vehicle or 10ng/mL TGFB for 3 hours.
|
Growth protocol |
SUM159 and WHIM12 cells were grown in the F12, (Gibco) and MEM (Gibco), respectively, and split 1:3 every two days.
|
Extracted molecule |
total RNA |
Extraction protocol |
Total RNA was isolated using Sigma GenElute RNA Isolation Kit
|
Label |
Biotin
|
Label protocol |
The WT Expression HT Kit for Robotics (Ambion) was used to generate sense-strand cDNA from total RNA. Following synthesis of sense-strand cDNA, the cDNA was fragmented and labeled with the Affymetrix GeneChip HT Terminal Labeling Kit.
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Hybridization protocol |
Fragmented and labeled cDNA was used to prepare a hybridization cocktail with the Affymetrix GeneTitan Hybridization Wash and Stain Kit for WT Arrays.
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Scan protocol |
Hybridization, washing, staining and scanning of the Affymtrix peg plate arrays was carried out using the Affymetrin GeneTitan MC Instrument.
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Description |
Gene expression data from control transfected and vehicle treated WHIM12 cells
|
Data processing |
Affymetrix Expression Console Software was used for basic data analysis and quality control (RMA normalization).
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Submission date |
May 04, 2015 |
Last update date |
Nov 10, 2015 |
Contact name |
Angelique Whitehurst |
E-mail(s) |
angelique.whitehurst@utsouthwestern.edu
|
Phone |
214-645-6066
|
Organization name |
UT Southwestern
|
Department |
Simmons Cancer Center
|
Street address |
5323 Harry Hines Blvd.
|
City |
Dallas |
State/province |
TX |
ZIP/Postal code |
75390-8807 |
Country |
USA |
|
|
Platform ID |
GPL11532 |
Series (2) |
GSE63984 |
Expression data to identify putative transcriptional targets of ZNF165 in Triple Negative Breast Cancer (TNBC) |
GSE63986 |
Integrative Functional Characterization of Cancer-Testis Antigens Defines Obligate Participation in Multiple Hallmarks of Cancer |
|