|
| Status |
Public on May 13, 2015 |
| Title |
Input |
| Sample type |
SRA |
| |
|
| Source name |
K14CreER SmoM2 cells
|
| Organism |
Mus musculus |
| Characteristics |
cell type: K14CreER SmoM2 cells
chip antibody: none (Input DNA)
|
| Treatment protocol |
Mice were injected with 2.5mg of tamoxifen for 10 consecutive days and sacrifice 9 weeks after the first injection. Tail epidermis was digested in trypsin and single cells were stained with the appropriate antibodies and FACS sorted before RNA extraction.
|
| Growth protocol |
Mice were maintained in a certified animal house according to European guidelines
|
| Extracted molecule |
genomic DNA |
| Extraction protocol |
ChIP was performed using Millipore EZ-Magna ChIP kit.
Between 5-10ng of qPCR verified ChIPed DNA or input DNA was processed with the TruSeq ChIP Sample Prep Kit (Illumina) according to manufacturer instruction.
|
| |
|
| Library strategy |
ChIP-Seq |
| Library source |
genomic |
| Library selection |
ChIP |
| Instrument model |
Illumina HiScanSQ |
| |
|
| Data processing |
ChIP-seq reads were aligned to the mm9 genome assembly using BWA software.
Peaks were identified using MACS software using a FDR treshold of 5% and a minimal enrichment of 2.
Genome_build: mm9
Supplementary_files_format_and_content: wig file
|
| |
|
| Submission date |
May 11, 2015 |
| Last update date |
May 15, 2019 |
| Contact name |
Jean-Christophe Larsimont |
| E-mail(s) |
jclarsim@gmail.com
|
| Organization name |
Université Libre de Bruxelles (ULB)
|
| Department |
Faculté de médecine
|
| Lab |
Institut de Recherche Interdisciplinaire en Biologie Humaine et Moléculaire (IRIBHM)
|
| Street address |
808, route de Lennik
|
| City |
Brussels |
| State/province |
Brussels |
| ZIP/Postal code |
1070 |
| Country |
Belgium |
| |
|
| Platform ID |
GPL16173 |
| Series (2) |
| GSE68755 |
Sox9 controls self-renewal of oncogene targeted cells and links tumor initiation and invasion [ChIP-Seq] |
| GSE68756 |
Sox9 controls self-renewal of oncogene targeted cells and links tumor initiation and invasion |
|
| Relations |
| BioSample |
SAMN03652362 |
| SRA |
SRX1023382 |
