|
| Status |
Public on Sep 17, 2016 |
| Title |
CPEB_overexp_1 |
| Sample type |
RNA |
| |
|
| Source name |
lentivirus-transduced HFF cells
|
| Organism |
Homo sapiens |
| Characteristics |
cell type: HFF cells
lentivirus transduction: CPEB-expressing construct
time: 5d-post-transduction
|
| Treatment protocol |
HCMV infections were performed as described in Stark et al., 2012 and Belzile et al., 2014. For lentivirus transductions (GFP and CPEB expression cells), puromycin drug selection was initiated at 48 h, and cells were harvested 3 days subsequently.
|
| Growth protocol |
HFFs and ES-derived NPCs were cultured as described in Sanders et al., 2008 and in Belzile et al., 2014.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
TRIzol (Invitrogen/Life Technologies) extraction of RNA, according to the manufacturer’s instructions.
|
| Label |
biotin
|
| Label protocol |
WT Terminal Labeling Kit, Affymetrix
|
| |
|
| Hybridization protocol |
Hybridization cocktails containing ~5.5 ug of fragmented and labeled DNA target were prepared and applied to GeneChip Human Splice Junction arrays. Hybridization was performed for 16 hours using the Fluidics 450 station.
|
| Scan protocol |
Arrays were scanned using the Affymetrix 3000 7G scanner and GeneChip Operating Software version 1.4 to produce .CEL intensity files.
|
| Description |
Array Sample 16
|
| Data processing |
Data processed using Affymetrix package (Affy Power Tools) apt-probeset-summarize. Iter-plier algorithm used to quantify probesets. As previously described (Huelga et al., 2012).
HJAY_r2.pgf
|
| |
|
| Submission date |
Oct 08, 2015 |
| Last update date |
Sep 17, 2016 |
| Contact name |
Gene Yeo |
| E-mail(s) |
geneyeo@ucsd.edu
|
| Organization name |
UCSD
|
| Street address |
2880 Torrey Pines Scenic Dr. Room 3805/Yeo Lab
|
| City |
La Jolla |
| State/province |
CA |
| ZIP/Postal code |
92037 |
| Country |
USA |
| |
|
| Platform ID |
GPL15106 |
| Series (2) |
| GSE73843 |
Transcriptome analysis of diverse cell types infected with human cytomegalovirus [array] |
| GSE74250 |
Transcriptome analysis of diverse cell types infected with human cytomegalovirus |
|
