|
| Status |
Public on Dec 01, 2015 |
| Title |
10608 |
| Sample type |
SRA |
| |
|
| Source name |
whole blood
|
| Organism |
Homo sapiens |
| Characteristics |
Sex: female
age_category: mid
case/control status: case
|
| Extracted molecule |
total RNA |
| Extraction protocol |
total RNA was extracted from whole blood using the Norgen protocol; small RNA was size fractionated from total RNA using polyacrylamide gel
small RNA was used as the starting material to generate small RNA libraries following the Illumina TruSeq Small RNA protocol
|
| |
|
| Library strategy |
miRNA-Seq |
| Library source |
transcriptomic |
| Library selection |
size fractionation |
| Instrument model |
Illumina HiSeq 1000 |
| |
|
| Data processing |
Adapters were trimmed using cutadapt
After adapter trimming, sequences shorter than 14 nucleotides were discarded.
Trimmed reads were aligned to the miRbase v.21 using SHRiMP aligner; only reads with a minimum average PHRED score of 10 were considered for alignment
Genome_build: miRBase v.21
Supplementary_files_format_and_content: raw counts in .txt format
|
| |
|
| Submission date |
Oct 19, 2015 |
| Last update date |
May 15, 2019 |
| Contact name |
Aliza P. Wingo |
| Organization name |
Atlanta VAMC/Emory University
|
| Department |
Psychiatry
|
| Street address |
1670 Clairmont Rd
|
| City |
Decatur |
| State/province |
GA |
| ZIP/Postal code |
30033 |
| Country |
USA |
| |
|
| Platform ID |
GPL15433 |
| Series (1) |
| GSE74162 |
genome-wide profiling of microRNA in PTSD with comorbid depression (PTSD&Dep) |
|
| Relations |
| BioSample |
SAMN04195129 |
| SRA |
SRX1355569 |
