|
| Status |
Public on Jul 15, 2020 |
| Title |
CPC5 rep8 |
| Sample type |
SRA |
| |
|
| Source name |
plasma
|
| Organism |
Homo sapiens |
| Characteristics |
tissue: plasma
cerebral performance category score: CPC5
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was extracted from a total of 400 μl of plasma, then 6ul of total RNA was use to prepare short RNA NGS library using NEBNEXT library generation kit
RNA libraries were prepared for sequencing using standard Illumina protocols
|
| |
|
| Library strategy |
miRNA-Seq |
| Library source |
transcriptomic |
| Library selection |
size fractionation |
| Instrument model |
Illumina NextSeq 500 |
| |
|
| Data processing |
Sequenced reads were trimmed for adaptor sequence, then mapped to miRbase 20
Expression levels are measured as Tags Per Million (TPM). The number of reads for a particular microRNA is divided by the total number of mapped reads and multiplied by 1 million (Tags Per Million). The differential expression analysis is done using the EdgeR statistical software package
Genome_build: miRbase 20
Supplementary_files_format_and_content: tsv (tab-separated values) text files contain counts and TPM of microRNAs for each sample
|
| |
|
| Submission date |
Oct 20, 2015 |
| Last update date |
Jul 15, 2020 |
| Contact name |
Yvan Devaux |
| E-mail(s) |
yvan.devaux@lih.lu
|
| Organization name |
LIH
|
| Lab |
Cardiovascular Research Unit
|
| Street address |
120 route d'Arlon
|
| City |
Luxembourg |
| ZIP/Postal code |
1150 |
| Country |
Luxembourg |
| |
|
| Platform ID |
GPL18573 |
| Series (1) |
| GSE74198 |
Next generation sequencing identifies circulating microRNAs in cardiac arrest patients |
|
| Relations |
| BioSample |
SAMN04196702 |
| SRA |
SRX1357347 |
