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Sample GSM1974556

Query DataSets for GSM1974556

Status

Public on Jan 13, 2016

Title

Input Human Brain PrecentralGyrus HS1

Sample type

SRA

Source name

Human female precentral gyrus tissue

Organism

Homo sapiens

Characteristics

tissue: Precentral gyrus
gender: Female
age: 68
Cause of death: Myocardial decompensation with sepsis
postmortem delay: 4.2 hrs
experiment type: Input
chip antibody: none

Extracted molecule

genomic DNA

Extraction protocol

Crosslink, Lyse and Immunoprecipitate
Illumina Truseq DNA library protocol

Library strategy

ChIP-Seq

Library source

genomic

Library selection

ChIP

Instrument model

Illumina NextSeq 500

Data processing

Mapping Illumina: Bowtie (1.1.0), parameters: -m 1 --best --strata --chunkmbs 512 -S --phred64-quals -q -l 40/45 -n 1
Mapping SOLiD: Bowtie (1.1.0), parameters: -m 1 --best --strata --chunkmbs 512 -S -l 50 -n 3 -C -Q -f
Peak calling: MACS2 (2.1.0), parameters: callpeak -B -p 1e-5 -g hs --nomodel --extsize 400 --llocal 100000
genome build: hg38, rheMac3, panTro4, mm10
processed data files format and content: txt files contain called peaks
processed data files format and content: narrowPeak files contain called peaks in BED format
processed data files format and content: bedGraph files contain treat pileup
processed data files format and content: bw files contain treat pileup read per million (RPM) normalized in bigWig format

Submission date

Dec 17, 2015

Last update date

May 15, 2019

Contact name

Menno P Creyghton

E-mail(s)

m.creyghton@hubrecht.eu

Organization name

Hubrecht Institute

Lab

Creyghton