|
| Status |
Public on Jan 20, 2017 |
| Title |
6hr_UC_PO |
| Sample type |
RNA |
| |
|
| Channel 1 |
| Source name |
stimulated by 4µg/mL recombinant human periostin for 6 hrs
|
| Organism |
Canis lupus familiaris |
| Characteristics |
cell line: Canine keratinocyte cell line (CPEK)
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted from cell cultures using the RNeasy Plus Micro Kit (Qiagen, Hilden, Germany).
|
| Label |
Cy5
|
| Label protocol |
Agilent two-color Low Input Quick Amp Labeling Kit labeling protocol
|
| |
|
| Channel 2 |
| Source name |
unstimulated control
|
| Organism |
Canis lupus familiaris |
| Characteristics |
cell line: Canine keratinocyte cell line (CPEK)
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted from cell cultures using the RNeasy Plus Micro Kit (Qiagen, Hilden, Germany).
|
| Label |
Cy3
|
| Label protocol |
Agilent two-color Low Input Quick Amp Labeling Kit labeling protocol
|
| |
|
| |
| Hybridization protocol |
Agilent two-color gene expression hyb/wash protocol
|
| Scan protocol |
Microarray slides were scanned in an Agilent SureScan G4900DA at 5 micron resolution. Images werequantified using Agilent Feature Extraction software version 11.5.1.1
|
| Description |
Sampling time_6hr
|
| Data processing |
LOWESS normalized, background subtracted VALUE data obtained from log10 of processed Red signal/processed Green signal. Agilent software was used.
|
| |
|
| Submission date |
Jan 20, 2016 |
| Last update date |
Jan 20, 2017 |
| Contact name |
TAKAYUKI MINESHIGE |
| E-mail(s) |
dv1206@azabu-u.ac.jp
|
| Organization name |
Azabu University
|
| Street address |
Chuo-ku, Kyowa 4-22-6
|
| City |
Sagamihara |
| State/province |
Kanagawa |
| ZIP/Postal code |
00000 |
| Country |
Japan |
| |
|
| Platform ID |
GPL15379 |
| Series (1) |
| GSE77041 |
Canine keratinocyte cell line: Unstimulated control (UC) vs. Samples stimulated by recombinant periostin (PO). |
|
