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Sample GSM2051500 Query DataSets for GSM2051500
Status Public on May 04, 2016
Title HOP-92
Sample type genomic
 
Channel 1
Source name Cultured Cell Line
Organism Homo sapiens
Characteristics cell line: HOP-92
Growth protocol Individual cell lines were cultured according to source guidelines
Extracted molecule genomic DNA
Extraction protocol Genomic DNA extracted using Qiagen Dneasy Blood and Tissue Kit, according the manufacturer's instructions
Label Cy5
Label protocol 250ng of purified genomic DNA was labeled with Cy-labeled primers using Exo- Klenow, according to the Roche Nimblegen dual color labeling protocol, with modifications
 
Channel 2
Source name pooled genomic DNA from 40 healthy males
Organism Homo sapiens
Characteristics sample type: pooled genomic DNA from 40 healthy males
Growth protocol Individual cell lines were cultured according to source guidelines
Extracted molecule genomic DNA
Extraction protocol Genomic DNA extracted using Qiagen Dneasy Blood and Tissue Kit, according the manufacturer's instructions
Label Cy3
Label protocol 250ng of purified genomic DNA was labeled with Cy-labeled primers using Exo- Klenow, according to the Roche Nimblegen dual color labeling protocol, with modifications
 
 
Hybridization protocol Oligoarray control targets and hybridization buffer were added, and samples were applied to 2x400K microarrays enclosed in Agilent SureHyb-enabled hybridization chambers. After hybridization, slides were washed according to the manufacturer's instructions
Scan protocol Scanned on an Agilent G2565A scanner.
Data processing Images were quantified using Agilent Feature Extraction Software (version 11), normalized log2 ratios were generated by OmicSoft ArrayStudio V8.1
 
Submission date Jan 29, 2016
Last update date May 04, 2016
Contact name Dan Baker
E-mail(s) bakerd@amgen.com
Phone 805-447-1000
Organization name Amgen Inc.
Department Molecular Sciences
Street address 1 Amgen Center Drive
City Thousand Oaks
State/province CA
ZIP/Postal code 91320
Country USA
 
Platform ID GPL19387
Series (1)
GSE77414 Investigation of MET copy number in various cancer cell lines

Data table header descriptions
ID_REF
VALUE normalized log2 ratio (Cy5/Cy3) representing test/reference

Data table
ID_REF VALUE
A_16_P17991140 0.8533
A_16_P33811607 -0.1805
A_16_P21504511 0.2901
A_16_P35765557 0.5526
A_18_P22657524 -0.3528
A_16_P16173769 0.0306
A_16_P20508688 -0.815
A_16_P17090144 0.3011
A_16_P20531565 -0.2173
A_16_P38660011 -0.5055
A_18_P13399885 0.1656
A_18_P10796304 0.3925
A_16_P03203060 -0.2854
A_16_P03779402 0.3872
A_14_P137194 0.3295
A_18_P14055431 0.0561
A_16_P15776094 -0.4076
A_16_P20147195 -0.771
A_16_P01289071 0.0447
A_16_P38098484 0.7959

Total number of rows: 410753

Table truncated, full table size 8899 Kbytes.




Supplementary file Size Download File type/resource
GSM2051500_HOP-92.txt.gz 117.2 Mb (ftp)(http) TXT
Processed data provided as supplementary file

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