|
| Status |
Public on May 04, 2016 |
| Title |
MKN-45 |
| Sample type |
genomic |
| |
|
| Channel 1 |
| Source name |
Cultured Cell Line
|
| Organism |
Homo sapiens |
| Characteristics |
cell line: MKN-45
|
| Growth protocol |
Individual cell lines were cultured according to source guidelines
|
| Extracted molecule |
genomic DNA |
| Extraction protocol |
Genomic DNA extracted using Qiagen Dneasy Blood and Tissue Kit, according the manufacturer's instructions
|
| Label |
Cy5
|
| Label protocol |
250ng of purified genomic DNA was labeled with Cy-labeled primers using Exo- Klenow, according to the Roche Nimblegen dual color labeling protocol, with modifications
|
| |
|
| Channel 2 |
| Source name |
pooled genomic DNA from 40 healthy males
|
| Organism |
Homo sapiens |
| Characteristics |
sample type: pooled genomic DNA from 40 healthy males
|
| Growth protocol |
Individual cell lines were cultured according to source guidelines
|
| Extracted molecule |
genomic DNA |
| Extraction protocol |
Genomic DNA extracted using Qiagen Dneasy Blood and Tissue Kit, according the manufacturer's instructions
|
| Label |
Cy3
|
| Label protocol |
250ng of purified genomic DNA was labeled with Cy-labeled primers using Exo- Klenow, according to the Roche Nimblegen dual color labeling protocol, with modifications
|
| |
|
| |
| Hybridization protocol |
Oligoarray control targets and hybridization buffer were added, and samples were applied to 2x400K microarrays enclosed in Agilent SureHyb-enabled hybridization chambers. After hybridization, slides were washed according to the manufacturer's instructions
|
| Scan protocol |
Scanned on an Agilent G2565A scanner.
|
| Data processing |
Images were quantified using Agilent Feature Extraction Software (version 11), normalized log2 ratios were generated by OmicSoft ArrayStudio V8.1
|
| |
|
| Submission date |
Jan 29, 2016 |
| Last update date |
May 04, 2016 |
| Contact name |
Dan Baker |
| E-mail(s) |
bakerd@amgen.com
|
| Phone |
805-447-1000
|
| Organization name |
Amgen Inc.
|
| Department |
Molecular Sciences
|
| Street address |
1 Amgen Center Drive
|
| City |
Thousand Oaks |
| State/province |
CA |
| ZIP/Postal code |
91320 |
| Country |
USA |
| |
|
| Platform ID |
GPL19387 |
| Series (1) |
| GSE77414 |
Investigation of MET copy number in various cancer cell lines |
|
