|
| Status |
Public on Feb 10, 2017 |
| Title |
HM1-hiPSC-Plan B |
| Sample type |
SRA |
| |
|
| Source name |
induced pluripotent stem cell
|
| Organism |
Homo sapiens |
| Characteristics |
cell line/strain: induced pluripotent stem cell
cell type: induced pluripotent stem cell
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was harvested using Trizol reagent. Illumina TruSeq RNA Sample Prep Kit (Cat#FC-122-1001) was used with 1 ug of total RNA for the construction of sequencing libraries.
RNA libraries were prepared for sequencing using standard Illumina protocols
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina NextSeq 500 |
| |
|
| Data processing |
Illumina Casava1.7 software used for basecalling.
Sequenced reads were trimmed for adaptor sequence, and masked for low-complexity or low-quality sequence, then mapped to mm10 whole genome using bowtie
Transcripts Per Million were calculated using a protocol from RSEM
Genome_build: hg19
Supplementary_files_format_and_content: TPM
|
| |
|
| Submission date |
Mar 11, 2016 |
| Last update date |
May 15, 2019 |
| Contact name |
qianyu chen |
| E-mail(s) |
chen_qianyu@gibh.ac.cn
|
| Phone |
+8602032015236
|
| Organization name |
Guangzhou Institutes of Biomedicine and Health,Chinese Academy of Sciences
|
| Lab |
Guangjin Pan
|
| Street address |
190 Kai Yuan Avenue, Science Park
|
| City |
Guangzhou |
| ZIP/Postal code |
510530 |
| Country |
China |
| |
|
| Platform ID |
GPL18573 |
| Series (1) |
| GSE79134 |
Optimized Approaches for Generation of Integration-free iPSCs from Human Urine-derived Cells with Small Molecules and Autologous Feeder |
|
| Relations |
| BioSample |
SAMN04546653 |
| SRA |
SRX1629340 |
