|
Status |
Public on Feb 06, 2018 |
Title |
MCF7-N small RNA-seq |
Sample type |
SRA |
|
|
Source name |
Normoxic MCF7 breast cancer cells
|
Organism |
Homo sapiens |
Characteristics |
cell line: MCF7 cell type: epithelial
|
Treatment protocol |
1% hypoxia treatment for 18 hrs
|
Growth protocol |
culture in DMEM medium
|
Extracted molecule |
total RNA |
Extraction protocol |
Trizol based RNA extraction method Small RNA libraries were constructed using the NEBNext Small RNA Library prep kit.
|
|
|
Library strategy |
miRNA-Seq |
Library source |
transcriptomic |
Library selection |
size fractionation |
Instrument model |
Illumina NextSeq 500 |
|
|
Data processing |
mapping with Bowtie read counting with Homer Genome_build: hg19 Supplementary_files_format_and_content: raw count CSV
|
|
|
Submission date |
Mar 31, 2016 |
Last update date |
May 15, 2019 |
Contact name |
Max Chang |
E-mail(s) |
mchang@ucsd.edu
|
Organization name |
University of California, San Diego
|
Street address |
9500 Gilman Drive
|
City |
La Jolla |
State/province |
CA |
ZIP/Postal code |
92093 |
Country |
USA |
|
|
Platform ID |
GPL18573 |
Series (2) |
GSE79787 |
small RNA sequencing for identifying hypoxic responsive miRNAs |
GSE79789 |
miR25/93 mediates the hypoxia-induced immunosuppression by repression of cGAS |
|
Relations |
BioSample |
SAMN04594908 |
SRA |
SRX1671938 |