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Status |
Public on Aug 15, 2016 |
Title |
BelTatar_65 |
Sample type |
genomic |
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|
Source name |
Belarusian Lipka Tatars_blood
|
Organism |
Homo sapiens |
Characteristics |
population: Belarusian Lipka Tatars region: Belarus tissue: blood
|
Extracted molecule |
genomic DNA |
Extraction protocol |
Extraction of genomic DNA from blood was performed using standard extraction protocols.
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Label |
Cy3, Cy5
|
Label protocol |
200ng of genomic DNA was whole-genome amplified in an overnight reaction at 37°C using amplification master mix (WG-AMM) and primer/neutralization mix (WG-MP1). After incubation the amplified DNA was fragmented with fragmentation mix (WG-FRG), precipitated with isopropanol and precipitation mix (PA1) and resuspended in hybridization buffer (RA1).
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Hybridization protocol |
RA1 resuspended DNA was loaded onto BeadChips arrays. After overnight incubation at 48°C, single-base extension and allele-specific staining was performed on a Teflow chamber rack system (Tecan, Maennedorf, Switzerland).
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Scan protocol |
After allele-specific staining BeadChip arrays were coated with XC4/ethanol, dried for 1 hour and scanned on a BeadArray Reader (Illumina).
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Description |
Genomic DNA extracted from blood was genotyped using HumanOmniExpress-24 v1.0 BeadChip (Illumina).
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Data processing |
Image data was analyzed using Genomestudio v2011.1 with GT module 1.9.4 (Illumina).
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Submission date |
Jun 06, 2016 |
Last update date |
Aug 15, 2016 |
Contact name |
Tuuli Reisberg |
Organization name |
University of Tartu
|
Street address |
Riia 23
|
City |
Tartu |
ZIP/Postal code |
51010 |
Country |
Estonia |
|
|
Platform ID |
GPL21168 |
Series (1) |
GSE82309 |
East Eurasian ancestry in the middle of Europe: genetic footprints of Steppe nomads in the genomes of Belarusian Lipka Tatars |
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