|
| Status |
Public on Jan 26, 2020 |
| Title |
TBX5-KO2_rep1(IonXpressRNA_003) |
| Sample type |
SRA |
| |
|
| Source name |
iPSC-CM_TBX5-KO2
|
| Organism |
Homo sapiens |
| Characteristics |
differentiation status: Day 30 post differentiation
cell type: iPSC-CM
genotype: TBX5-KO
|
| Treatment protocol |
CM differentiation (small molecule approach)
|
| Growth protocol |
E8
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA mini kit (Qiagen)
mRNA was isolated from total RNA (500ng) using the Ambion's mRNA direct kit (Dynabeads® mRNA Purification Kit ). Libraries were prepared with the Ion Total RNA-Seq Kit v2 according to the manufacturer's instructions. ERCC were spiked-in before the mRNA isolation.
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Ion Torrent PGM |
| |
|
| Data processing |
WT pipeline for Ion Torrent - 1.0
Partek Flow software
Genome_build: hg19
Supplementary_files_format_and_content: excel file with Differential expression analysis results, generated by Partek Flow
|
| |
|
| Submission date |
Jun 23, 2016 |
| Last update date |
Jan 26, 2020 |
| Contact name |
Joseph Wu |
| Organization name |
Stanford University School of Medicine
|
| Street address |
Stanford University School of Medicine
|
| City |
Stanford |
| State/province |
Carlifornia |
| ZIP/Postal code |
94305 |
| Country |
USA |
| |
|
| Platform ID |
GPL17301 |
| Series (1) |
|
| Relations |
| BioSample |
SAMN05289920 |
| SRA |
SRX1873045 |
