|
| Status |
Public on Jan 22, 2024 |
| Title |
T2DM201B |
| Sample type |
RNA |
| |
|
| Source name |
skeletal muscle, T2DM, diet-induced weight loss before surgery
|
| Organism |
Homo sapiens |
| Characteristics |
phenotype: obese
disease status: type 2 diabetes mellitus
tissue: skeletal muscle (vastus lateralis)
treatment: Roux-en-Y gastric bypass surgery
timepoint: before surgery, after diet-induced weight loss
individual identifier: 201
|
| Treatment protocol |
We sampled skeletal muscle biopsies from vastus lateralis with a Bergstrom needle using suction and local anaesthesia (1% lidocaine) at both visits (B and C). Biopsies were snap-frozen in liquid nitrogen and stored at -80°C subsequently.
|
| Growth protocol |
16 severely obese patients without type 2 diabetes mellitus (OB) and nine severely obese patients with type 2 diabetes mellitus (T2DM) were elected for Roux-en-Y gastric bypass surgery. We examined the participants after a diet-induced weight loss (B) and approximately 4 months post-surgery (C).
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted from approximately 20 mg of muscle tissue using Trizol (Invitrogen).
|
| Label |
SYBR green
|
| Label protocol |
30 ng RNA was reverse transcribed in 30 μl reactions using the miRCURY LNA Universal RT microRNA PCR, Polyadenylation and cDNA synthesis kit (Exiqon). cDNA was diluted 100x and assayed in 10 ul PCR reactions according to the protocol; each microRNA was assayed once by qPCR on the microRNA Ready-to-Use PCR, Human Panel I using ExiLENT SYBR® Green master mix. Negative controls excluding template from the reverse transcription reaction was performed and profiled like the samples. The amplification was performed in a LightCycler® 480 Real-Time PCR System (Roche) in 384-well plates.
|
| |
|
| Hybridization protocol |
n/a
|
| Scan protocol |
n/a
|
| Description |
Sample name = 66
|
| Data processing |
Non-normalized data: Background filtered Cq-values.
Normalized data: All data was normalised to the average of assays detected in all samples (dCq = global mean Cq – assay Cq (sample)).
Fold-change data: 2^dCq
|
| |
|
| Submission date |
Sep 20, 2016 |
| Last update date |
Jan 22, 2024 |
| Contact name |
Malene Maag Kristensen |
| E-mail(s) |
malenemk@hotmail.com
|
| Organization name |
University of Copenhagen
|
| Street address |
Blegdamsvej 3
|
| City |
Copenhagen |
| ZIP/Postal code |
2200 |
| Country |
Denmark |
| |
|
| Platform ID |
GPL22463 |
| Series (1) |
| GSE87131 |
Weight loss following Roux-en-Y gastric bypass surgery leads to changes in the expression of several miRNAs and their predicted target genes in skeletal muscle |
|
