|
| Status |
Public on May 22, 2017 |
| Title |
SFC841-03-02 [SNP genotype] |
| Sample type |
genomic |
| |
|
| Source name |
iPS cell line derived from dermal fibroblasts, control donor 841, clone 2
|
| Organism |
Homo sapiens |
| Characteristics |
gender: male
cell type: induced pluripotent stem (iPS) cell line derived from dermal fibroblasts
culture conditions: mTeSR-1 + matrigel
|
| Treatment protocol |
untreated cell samples; iPSc lines all grown in mTeSR-1 medium + ES-qualified matrigel prior to harvest
|
| Extracted molecule |
genomic DNA |
| Extraction protocol |
genomic DNA was extracted using QIAGEN DNeasy blood and tissue kit
|
| Label |
Biotin (C, G), Dinitrophenyl (A, T)
|
| Label protocol |
Standard Illumina labelling protocol
|
| |
|
| Hybridization protocol |
Standard Illumina hybridisation protocol
|
| Scan protocol |
Standard Illumina scanning protocol for BeadChip Arrays
|
| Description |
single replicate
cluster file: humanomniexpress_24v1-0_a
manifest: humanomniexpress_24v1-0_a
|
| Data processing |
The scanned data files were processed by GenomeStudio Genotyping module v1.9, using the cluster file and manifest HumanCytoSNP-12v2-1_H provided by Illumina for GPL13829, and humanomniexpress_24v1-0_a for GPL19699. Raw data was also uploaded to KaryoStudio (Illumina) for generation of karyotype reports (see publication).
|
| |
|
| Submission date |
May 21, 2017 |
| Last update date |
May 22, 2017 |
| Contact name |
Sally A Cowley |
| E-mail(s) |
sally.cowley@path.ox.ac.uk
|
| Phone |
+44 (0) 1865 275600
|
| Organization name |
University of Oxford
|
| Department |
Sir William Dunn School of Pathology
|
| Lab |
James Martin Stem Cell Facility
|
| Street address |
South Parks Road
|
| City |
Oxford |
| ZIP/Postal code |
OX1 3RE |
| Country |
United Kingdom |
| |
|
| Platform ID |
GPL21168 |
| Series (2) |
| GSE99124 |
SNP genotype data from induced Pluripotent Stem cells from healthy control donors |
| GSE99125 |
Gene Expression and SNP genotype data from induced Pluripotent Stem cells from healthy control donors |
|
