|
| Status |
Public on May 30, 2008 |
| Title |
Hypothalamus-Mecp2-null C57BL/6J-biological rep4 |
| Sample type |
RNA |
| |
|
| Source name |
Hypothalamus, Mecp2-null
|
| Organism |
Mus musculus |
| Characteristics |
genotype: Mecp2-null
gender: male
age: 6 weeks
strain: C57BL/6J
strain: C57BL/6J
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted from the hypothalamus of 6 weeks old male mice using TRIzol reagent, DNaseI treated, and purified using the RNeasy mini kit, all according to the manufacturers protocols
|
| Label |
biotin
|
| Label protocol |
Total RNA was reverse transcribed to produce double-stranded cDNA. The cDNA product was used as a template for the in vitro transcription reaction, producing biotin-labeled
|
| |
|
| Hybridization protocol |
Following fragmentation of 15.0 mg of the labeled cRNA, a hybridization cocktail containing Affymetrix spike-in controls and fragmented labeled cRNA was loaded onto a GeneChip array. The array was hybridized 16 hours
|
| Scan protocol |
The array was stained using the EukGEws2v5 protocol on the Fluidics Station 450. The stained array was scanned on the Affymetrix GeneChip Scanner 3000.
|
| Description |
Gene expression data
|
| Data processing |
The images were analyzed and quality control metrics recorded using Affymetrix GCOS software version 1.1 and scale factor of 500. Subsequently, R based analysis using exonmap package and rma normalization
|
| |
|
| Submission date |
Apr 11, 2008 |
| Last update date |
May 23, 2008 |
| Contact name |
Maria Chahrour |
| E-mail(s) |
chahrour@bcm.tmc.edu
|
| Phone |
713-798-6695
|
| Organization name |
Baylor College of Medicine
|
| Department |
Molecular and Human Genetics
|
| Street address |
1 Baylor Plaza, T836
|
| City |
Houston |
| State/province |
TX |
| ZIP/Postal code |
77098 |
| Country |
USA |
| |
|
| Platform ID |
GPL6193 |
| Series (1) |
| GSE11150 |
Hypothalamic gene expression data from Mecp2-null and MECP2-transgenic mice |
|
