|
| Status |
Public on Jul 01, 2019 |
| Title |
OB_WT_2M_R1 |
| Sample type |
RNA |
| |
|
| Source name |
Olfactory bulb, WT, 2m
|
| Organism |
Mus musculus |
| Characteristics |
genotype/variation: WT
tissue: Olfactory bulb
gender: Female
age: 2 months
|
| Treatment protocol |
Not applicable.
|
| Growth protocol |
Not applicable.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
For olfactory bulb mRNA extraction, the Maxwell 16 simplyRNA kit (Promega) was used.
|
| Label |
biotin
|
| Label protocol |
The sense cDNA was prepared from 1 ng of total RNA using the Affymetrix GeneChip® WT Pico Kit (PN902623). The sense strand cDNA was then fragmented and biotinylated according to the manufacturer's instructions.
|
| |
|
| Hybridization protocol |
Labeled sense cDNA was hybridized to the GeneChip® Mouse Clariom S Arrays, washed and stained on a GeneChip® Fluidics 450 workstation (Affymetrix) according to the manufacturer protocols.
|
| Scan protocol |
GeneChips were scanned with the Affymetrix GeneChip® Scanner 3000 7G.
|
| Description |
WT_2M_T2_8682_S18
Affymetrix Mouse Clariom S chip with bioconductor (pd.clariom.s.mouse).
|
| Data processing |
Data were normalized with Bioconductor software using RMA algorithm.
|
| |
|
| Submission date |
Nov 07, 2017 |
| Last update date |
Jul 01, 2019 |
| Contact name |
Victor Segura |
| E-mail(s) |
vsegura@unav.es
|
| Organization name |
FIMA
|
| Department |
Unit of Proteomics, Genomics and Bioinformatics
|
| Street address |
Avda. Pío XII, 55
|
| City |
Pamplona |
| State/province |
Navarra |
| ZIP/Postal code |
31008 |
| Country |
Spain |
| |
|
| Platform ID |
GPL23038 |
| Series (1) |
| GSE106643 |
The olfactory molecular snapshot of Tg2576 mice at early stages of amyloid pathology |
|
