|
| Status |
Public on Dec 01, 2019 |
| Title |
UT3 |
| Sample type |
SRA |
| |
|
| Source name |
peripheral blood-derived mast cells
|
| Organism |
Homo sapiens |
| Characteristics |
treatment: Untreated
source: CD34+ derived
cell type: peripheral blood-derived mast cells
passages: >98% FcεRI+ CD117+, 10 weeks
genotype: WT
|
| Treatment protocol |
Human myeloma IgE (0.5 μg/ml) treatment for 18 h, anti-IgE (1 μg/ml) treatment for 2h after IgE treatment
|
| Growth protocol |
Peripheral blood-derived CD34+ cells were cultured with 5 ng/ml hIL-3, 100 ng/ml hSCF and 50 ng/ml hIL-6 for 3 weeks. Subsequently,100 ng/ml hSCF and 50 ng/ml hIL-6 were used for additional 7 weeks
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA libraries were prepared for sequencing using standard Illumina protocols
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina NextSeq 500 |
| |
|
| Data processing |
The sequenced reads were mapped tomm9 build of the human genome using STAR 2.5.1a with default parameters
FPKMs were calculated by Cufflink (2.2.1)
Genome_build: hg19
Supplementary_files_format_and_content: tab-delimited text files include RPKM values for each Sample.
|
| |
|
| Submission date |
Nov 24, 2017 |
| Last update date |
Dec 01, 2019 |
| Contact name |
Jingxian Zhang |
| E-mail(s) |
zhangjingxian.nus@gmail.com
|
| Organization name |
Institute of Molecular and Cell Biology (IMCB), A-Star, Singapore
|
| Department |
IMCB
|
| Street address |
61 Biopolis Drive Proteos
|
| City |
Singapore |
| State/province |
Singpaore |
| ZIP/Postal code |
138673 |
| Country |
Singapore |
| |
|
| Platform ID |
GPL18573 |
| Series (2) |
| GSE75527 |
Landscape of human mast cell chromatin: a rich resource for identification of novel mediators and genetic drivers of allergic and inflammatory diseases |
| GSE107316 |
Transcriptomic profiling of human CD34+ derived mast cells [RNA-Seq] |
|
| Relations |
| BioSample |
SAMN08097264 |
| SRA |
SRX3421758 |
