|
| Status |
Public on Feb 20, 2009 |
| Title |
VUMC_LGCIN1_b251495015494_s129_4 |
| Sample type |
genomic |
| |
|
| Channel 1 |
| Source name |
uterine cervix
|
| Organism |
Homo sapiens |
| Characteristics |
cervical intraepithelial neoplasia (CIN) lesion, grade 1
|
| Extracted molecule |
genomic DNA |
| Extraction protocol |
as described in Van zeeburg et al, Cancer Res, volume 65, p 1271-76, 2005
|
| Label |
Cy3
|
| Label protocol |
as described by Snijders et al, Nat Genetics, volume 29, p 263-4
|
| |
|
| Channel 2 |
| Source name |
Reference DNA
|
| Organism |
Homo sapiens |
| Characteristics |
Pooled DNA from the blood of 10 normal male individuals
|
| Extracted molecule |
genomic DNA |
| Extraction protocol |
as described in Wilting et al, J Pathol, volume 209, p 220-30
|
| Label |
Cy5
|
| Label protocol |
as ch1, Cy5 in stead of Cy3
|
| |
|
| |
| Hybridization protocol |
hybridisations were performed according to the manufacturer's instructions (Agilent Technologies)
|
| Scan protocol |
Microarray Scanner G2505B (Agilent Technologies), default settings
|
| Description |
CIN1 lesion, patient age 26 years, contains high risk Human Papilloma Virus
|
| Data processing |
Spots were quantified in Feature Extraction software version 9.5.1 (Agilent Technologies) using default settings.
|
| |
|
| Submission date |
Sep 19, 2008 |
| Last update date |
Feb 20, 2009 |
| Contact name |
Daoud Sie |
| E-mail(s) |
d.sie@vumc.nl
|
| Phone |
+31 20 4442428
|
| Organization name |
Vrije Universiteit Medical Center
|
| Department |
Pathology
|
| Lab |
Microarray Core Facility
|
| Street address |
De Boelelaan 1117
|
| City |
Amsterdam |
| ZIP/Postal code |
1081 HV |
| Country |
Netherlands |
| |
|
| Platform ID |
GPL5477 |
| Series (1) |
| GSE11573 |
Chromosomal signatures of a subset of high-grade premalignant cervical lesions closely resemble invasive carcinomas |
|
