|
| Status |
Public on Oct 01, 2008 |
| Title |
carcinomatous component |
| Sample type |
genomic |
| |
|
| Channel 1 |
| Source name |
carcinomatous component of a carcinosarcoma
|
| Organism |
Homo sapiens |
| Characteristics |
FFPE material, described in Vekony et al, Oral Oncology 2008 in press
carcinomatous component macrodissected
|
| Extracted molecule |
genomic DNA |
| Extraction protocol |
standard phenol/chloroform extraction
|
| Label |
Cy3
|
| Label protocol |
according to standard protocol ENZO BioArray™ CGH Labeling System (Farmingdale, NY, USA)
|
| |
|
| Channel 2 |
| Source name |
reference DNA
|
| Organism |
Homo sapiens |
| Characteristics |
Pooled DNA from the blood of 10 normal male individuals
|
| Extracted molecule |
genomic DNA |
| Extraction protocol |
as described in Wilting et al, J Pathol, volume 209, p 220-30
|
| Label |
Cy5
|
| Label protocol |
according to standard protocol ENZO BioArray™ CGH Labeling System (Farmingdale, NY, USA)
|
| |
|
| |
| Hybridization protocol |
hybridizations were performed according to the manufacturer's instructions (Agilent Technologies)
|
| Scan protocol |
Microarray Scanner G2565AB (Agilent Technologies), default settings
|
| Description |
500 ng of the carcinomatous component of a salivary gland carcinosarcoma has been hybridized to a 4x44k oligo array
|
| Data processing |
Spots were quantified in Feature Extraction software version 9.1 (Agilent Technologies) using default settings.
|
| |
|
| Submission date |
Sep 29, 2008 |
| Last update date |
Sep 30, 2008 |
| Contact name |
Daoud Sie |
| E-mail(s) |
d.sie@vumc.nl
|
| Phone |
+31 20 4442428
|
| Organization name |
Vrije Universiteit Medical Center
|
| Department |
Pathology
|
| Lab |
Microarray Core Facility
|
| Street address |
De Boelelaan 1117
|
| City |
Amsterdam |
| ZIP/Postal code |
1081 HV |
| Country |
Netherlands |
| |
|
| Platform ID |
GPL5477 |
| Series (1) |
| GSE12958 |
CGH analysis of two components of carcinosarcoma |
|
