|
| Status |
Public on Jan 04, 2021 |
| Title |
iMoP_sorted1 |
| Sample type |
RNA |
| |
|
| Source name |
mouse bone marrow, iMoP
|
| Organism |
Mus musculus |
| Characteristics |
strain: C57Bl/6
genotype: Cx3cr1+/gfp
|
| Treatment protocol |
no treatment
|
| Growth protocol |
animal facilities of the University of Freiburg under specific pathogen-free conditions
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Bones were flushed with full medium. Cells were extracted from bone marrow by magnetic cell separation and fluorescence-activated cell sorting. Total RNA was extracted from sorted cells with the Rneasy Micro Kit (Qiagen) as per manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Purified ds cDNA was fragmented using a combination of uracil DNA glycosylase (UDG) and apurinic/apyrimidinic endonuclease 1 (APE 1) at the dUTP residues followed by a terminal labeling with biotin.
|
| |
|
| Hybridization protocol |
Sample preparation for microarray hybridization was carried out as described in the Affymetrix GeneChip Pico Reagent Kit User Guide (Affymetrix, Inc., Santa Clara, CA, USA).
|
| Scan protocol |
Fluorescent signals were measured with an Affymetrix GeneChip Scanner 3000 7G. Fluidics and scan functions were controlled by the Affymetrix GeneChip Command Console v4.1.3 software.
|
| Description |
mouse bone marrow, iMoP
|
| Data processing |
Summarized probe set signals in log2 scale were calculated by using the GCCN-SST-RMA algorithm with the Affymetrix GeneChip Expression Console v1.4 Software. After exporting into Microsoft Excel, average signal values, comparison fold changes and significance P values were calculated. Probe sets with a fold change above 2.0 fold and a student’s t test P value lower than 0.05 were considered as significantly regulated.
|
| |
|
| Submission date |
Jul 20, 2018 |
| Last update date |
Jan 05, 2021 |
| Contact name |
Anne Loesslein |
| E-mail(s) |
anne.loesslein@uniklinik-freiburg.de
|
| Organization name |
Universität Freiburg
|
| Department |
CCI
|
| Street address |
Breisacherstr. 115
|
| City |
Freiburg |
| ZIP/Postal code |
79106 |
| Country |
Germany |
| |
|
| Platform ID |
GPL23038 |
| Series (1) |
| GSE117460 |
Genom-wide expression study of cMoP and iMoP from murine bone marrow |
|
